PHENOL SULPHURIC ACID CARBOHYDRATE ASSAY

REFERENCE: Anal. Chem. 28:(1956) 350-356.
 

OBJECTIVE:  To detect the presence of sugars. (Widely used method to monitor column eluates for elution of sugar containing compounds such as LPS, 0-antigen, etc.)
 

MATERIALS:


METHOD:

  1. Add 50 ul of 80% Phenol solution (80% Phenol by weight)
  2. Vortex. Add 2.0 ml concentrated Sulphuric Acid in a stream.
  3. Stand 10 min. at room temperature.
  4. Read absorbance an 490nm.



Updated 01 December 2000.