PHENOL SULPHURIC ACID CARBOHYDRATE
REFERENCE: Anal. Chem. 28:(1956) 350-356.
OBJECTIVE: To detect the presence of sugars.
(Widely used method to monitor column eluates for elution of sugar containing
compounds such as LPS, 0-antigen, etc.)
Standards:glucose 1 mg/ml stock solution
use 10, 20, 40, 60, 80 µl and make up each sample with
dH2O to a final volume of 100 µl.
Blank 100 µl water
Samples:50 µl (of 5 mg/ml LPS solution) and make up
to 100 µl.
Add 50 ul of 80% Phenol solution (80% Phenol by weight)
Vortex. Add 2.0 ml concentrated Sulphuric Acid in a stream.
Stand 10 min. at room temperature.
Read absorbance an 490nm.
Updated 01 December 2000.