Methods
If you use one of these methods, please use the associated reference where listed, or an online reference format (such as that shown below which is described in the Journal of Bacteriology January 1999 Instructions to Authors):
Hancock, R. E. W. September 19 1999, posting date. [Online.]
Hancock Laboratory Methods. Department of Microbiology and Immunology, University of British Columbia, British Columbia, Canada. [New website methods link] [xx xxxxxx xxxx, date last accessed.]
Displaying 97 Unique Methods
Antibiotics and Antimicrobial Peptides
Bacterial Genetics
- Alkaline Lysis Plasmid Miniprep
- Biparental Mating
- Blotting and Hybridization with Sodium Phosphate
- Blunt End Cloning of PCR Products
- CaCl2 Transformation of E. coli
- Chromosomal DNA Isolation
- Colony Blotting with 32P
- Colony Immunoblot
- Cosmid Library
- CsCl-Ethidium Bromide Gradient Plasmid Preparations
- DNA Isolation from Agarose Gels with DEAE Paper
- Grid for 100 Clones
- Isolation of Chromosomal DNA
- Isolation of Phage Resistant Mutants
- Lambda-TnphoA Mutagenesis of Cloned Genes in E. Coli
- M13 Single-Stranded Template Preparation
- Phage Resistant Mutant Isolation
- Quick Isolation of DNA from Agarose Gels
- Slot Lysis
- Southern Blot
- Transformation of Helicobacter Pylori
- Transposon Mutagenesis of Pseudomonas Aeruginosa
Protein Purification and Gel Electrophoresis
- Acetylation (or Succinylation) of Amino Groups on Proteins
- Acid-Urea Gel Electrophoresis
- Crosslinking of Intact Cells
- Crosslinking of Proteins
- Crosslinking of Whole Cells
- Electroblotting on to PVDF (Immobilon) Membrane
- Estimation of Amino Groups Using TNBS
- Isoelectric Focussing of Membrane Proteins by Slab Gel Method
- Isolation of Outer Membrane Protein from P. aeruginosa With Octyl-POE
- Isolation of Outer Membrane Protein With Zwittergent 3-14
- Lipid A Preparation
- Outer Membrane Protein Solubilization with Zwittergent 3-14
- Porin F Purification
- Protein and LPS Gels
- Protein Assay
- Protein Assay for Detergent Solubilized Proteins
- Separation of Outer and Inner Membranes
- Whole Cell Protein and LPS Gels
Liposome Methods
Outer Membranes
- Cell Envelope Preparation / Solubilization
- Crosslinking of Intact Cells
- Crosslinking of Proteins
- Crosslinking of Whole Cells
- Dansyl-Polymyxin Synthesis
- Dinitrophenylation Assay For Polymixin B
- French Press
- Inner Membrane Permeability Assay (ONPG Assay)
- KDO Assay
- Liposome Swelling Assay
- LPS Isolation (Darveau-Hancock Method)
- Methylation of Fatty Acids (Kropinski Method)
- Nitrocefin Assay on Whole Cells for Outer Membrane Permeability
- Nitrocefin Assay on Whole Cells for Outer Membrane Permeability Assessment
- NPN Uptake Assay / Flourescence Measurements
- Osmotic Shock Procedures
- Outer Membrane Preparation – One Step Sucrose Gradient Procedure
- Outer Membrane Protein Solubilization with Zwittergent 3-14
- Permeabilization of Whole Cells to Lysozyme
- Phase Partitioning of Cells
- Phosphate Uptake Assay
- Planar (Black) Lipid Bilayer Apparatus
- Planar Lipid Bilayer Experiment
- Planar Lipid Bilayer Experiment (Updated)
- Protease Digestion of Outer Membranes
- Synthesis of Dansyl-Polymyxin
Biochemical Assays
- Acetylation (or Succinylation) of Amino Groups on Proteins
- Alkaline Phosphatase and Phospholipase C Assays
- Alkaline Phosphatase C Assay for P. aeruginosa
- Carbohydrate Assays
- Dansyl-Polymyxin Synthesis
- Dehydrogenase Assays
- Dinitrophenylation Assay For Polymixin B
- Estimation of Amino Groups Using TNBS
- Glucosamine Rapid Assay
- KDO Assay
- Muramic Acid Assay
- Penicillin Binding Protein Assay
- Phenol Sulphuric Acid Carbohydrate Assay
- Phosphate Assay
- Phosphate Uptake Assay
- Whole Cell Phosphate Uptake Assay
Microbiological Techniques
Host-Pathogen Interactions
Functional Genomics
Immunology Based Methods
Peptides
Method Categories
- Antibiotics and Antimicrobial Peptides (6)
- Bacterial Genetics (22)
- Protein Purification and Gel Electrophoresis (18)
- Liposome Methods (6)
- Outer Membranes (26)
- Biochemical Assays (16)
- Microbiological Techniques (8)
- Host-Pathogen Interactions (3)
- Functional Genomics (1)
- Immunology Based Methods (8)
- Peptides (2)