Home | Contact | Site Map | Lab Location
Meeting Room Bookings | Lab Members Only

About Hancock | Research Areas | Methods | Publications | Staff | CMDR | Genome Annotation | OMP Annotation | Links | Career/Jobs


Hancock Laboratory : Refereed Journal Publications

Important:

            Most publications are copyrighted to their respective publishers. Refer the respective Journals for

                copyright information.

 

                To access pdf version of the publications, click the volume number link given. The publications are copyrighted to American Society for Microbiology (ASM). For ASM copyright and license information, go to http://www.journals.asm.org/misc/terms.shtml

           

The publications are listed in groups. Click the numbers to expand the publication details. Re-click to close the group
1-50  

1.Skurray, R.A., R.E.W. Hancock, and P. Reeves. 1974. Conmutants: class of mutants in Escherichia coliK-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage. J. Bacteriol. 119:726-735.

2.Hancock, R.E.W., and P. Reeves. 1975. Bacteriophage resistance in Escherichia coli K-12: General pattern of resistance. J. Bacteriol. 121:983-993.

3.Hancock, R.E.W., J.K. Davies, and P. Reeves. 1976. Cross resistance between bacteriophages and colicins in Escherichia coli K-12. J. Bacteriol. 126:1347-1350.

4.Hancock, R.E.W. and P. Reeves. 1976. Lipopolysaccharide-deficient, bacteriophage-resistant mutants of Escherichia coli K-12. J. Bacteriol. 127:98-108.

5.Hancock, R.E.W., and V. Braun. 1976. Nature of the energy requirement for the irreversible adsorption of bacteriophages T1 and f80 to Escherichia coli. J. Bacteriol. 12:409-415.

6.Hancock, R.E.W., and V. Braun. 1976. The colicin I receptor of Escherichia coli K-12 has a role in enterochelin-mediated iron transport. FEBS Lett. 65:208-210.

7.Braun, V., R.E.W. Hancock, K. Hantke, and A. Hartmann. 1976. Functional organization of the outer membrane of Escherichia coli. Phage and colicin receptors as components of iron uptake systems. J. Supramolecular Structure 5:37-58.

8.Hancock, R.E.W., K. Hantke, and V. Braun. 1976. Iron transport in Escherichia coli K-12: Involvement of the colicin B receptor and of a citrate-inducible protein. J. Bacteriol. 127:1370-1375.

9.Hancock, R.E.W., K. Hantke, and V. Braun. 1977. Iron transport in Escherichia coli K-12: 2,3-dihydroxybenzoate-promoted iron uptake. Arch. Microbiol. 114:231-239.

10.Hancock, R.E.W., and H. Nikaido. 1978. Outer membrane of Gram negative bacteria. XIX Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J. Bacteriol. 136:381-390.

11.Hancock, R.E.W., and G.M. Decad and H. Nikaido. 1979. Identification of the protein producing transmembrane diffusion pores in the outer membrane of Pseudomonas aeruginosa PA01. Biochim. Biophys. Acta. 554:323-331.

12.Hancock, R.E.W., and A.M. Carey. 1979. Outer membrane of Pseudomonas aeruginosa. Heat-and 2-mercaptoethanol-modifiable proteins. J. Bacteriol. 140:902-910.

13.Chen, U., R.E.W. Hancock, and R. Mishell. 1980. Mitogenic effects of purified outer membrane proteins from Pseudomonas aeruginosa. Infect. Immun. 28:178-184.

14.Hancock, R.E.W., and A.M. Carey. 1980. Protein D1 - a glucose-inducible, pore-forming protein from the outer membrane of Pseudomonas aeruginosa. FEMS Microbiol. Letters 8:105-109.

15.Nicas, T.I., and R.E.W. Hancock. 1980. Outer membrane protein H1 of Pseudomonas aeruginosa: Involvement in adaptive and mutational resistance to ethylenediamine tetraacetate, polymyxin B and gentamicin. J. Bacteriol. 143:872-878.

16.Hancock, R.E.W., R.T. Irvin, J.W. Costerton, and A.M. Carey. 1981. Pseudomonas aeruginosa outer membrane: peptidoglycan associated proteins. J. Bacteriol. 145:628-631.

17.Hancock, R.E.W., V.J. Raffle, and T.I. Nicas. 1981. Involvement of the outer membrane in gentamicin and streptomycin uptake and killing in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 19:777-785.

18.Benz, R., and R.E.W. Hancock. 1981. Properties of the large ion-permeable pores formed from protein F of Pseudomonas aeruginosa in lipid bilayer membranes. Biochim. Biophys. Acta 646:298-308.

19.Angus, B.L., A.M. Carey, D.A. Caron, A.M.B. Kropinski, and R.E.W. Hancock. 1982. Outer membrane permeability in Pseudomonas aeruginosa: Comparison of a wild-type with an antibiotic-supersusceptible mutant. Antimicrob. Agents Chemother. 21:299-309.

20.Kropinski, A.M.B., J. Kuzio, B.L. Angus, and R.E.W. Hancock. 1982. Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 21:310-319.

21.Hancock, R.E.W., K. Poole, and R. Benz. 1982. Outer membrane protein P of Pseudomonas aeruginosa: Regulation by phosphate deficiency and formation of small anion-specific channels in lipid bilayer membranes. J. Bacteriol. 150:730-38.

22.Hancock, R.E.W., A.A. Wieczorek, L.M. Mutharia, and K. Poole. 1982. Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: Isolation and characterization. Infect. Immun. 37:166-171.

23.Mutharia, L.M., T. I. Nicas, and R.E.W. Hancock. 1982. Outer membrane proteins of Pseudomonas aeruginosa serotype strains. J. Infect. Dis. 146:770-779.

24.Downum, K.R., R.E.W. Hancock, and G.H.N. Towers. 1982. Mode of action of alpha-terthienyl on Escherichia coli. Evidence for a photodynamic effect on membranes. Photochem. Photobiol. 36:517-523.

25.Nakajima, K., K. Muroga, and R.E.W. Hancock. 1983. Comparison of fatty acid, protein and serological properties distinguishing outer membranes of Pseudomonas anguilliseptica strains from those of fish pathogens and other Pseudomonads. Int. J. Systemat. Bacteriol. 33:1-8.

26.Nicas, T.I., and R.E.W. Hancock. 1983. Alteration of susceptibility to EDTA, polymyxin B and gentamicin in Pseudomonas aeruginosa by divalent cation regulation of outer membrane protein H1. J. Gen. Microbiol. 129:509-517.

27.Poole, K., and R.E.W. Hancock. 1983. Secretion of alkaline phosphatase and phospholipase C in Pseudomonas aeruginosa is specific and does not involve an increase in outer membrane permeability. FEMS Microbiol. Lett. 16:25-29.

28.Nicas, T.I., and R.E.W. Hancock. 1983. Pseudomonas aeruginosa outer membrane permeability: Isolation of a porin protein F-deficient mutant. J. Bacteriol. 153:281-285.

29.Darveau, R.P., and R.E.W. Hancock. 1983. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J. Bacteriol. 155:831-838.

30.Benz, R., M. Gimple, K. Poole and R.E.W. Hancock. 1983. An anion-selective channel from the Pseudomonas aeruginosa outer membrane. Biochim. Biophys. Acta 730:387-390.

31.Angus, B.L., and R.E.W. Hancock. 1983. Outer membrane porin proteins F, P and D1 of Pseudomonas aeruginosa and Pho E of Escherichia coli : Chemical crosslinking to reveal native oligomers. J. Bacteriol. 155:1042-1051.

32.Mutharia, L.M., and R.E.W. Hancock. 1983. Surface localization of Pseudomonas aeruginosa outer membrane porin protein F by using monoclonal antibodies. Infect. Immun. 42:1027-1033.

33.Hancock, R.E.W., L.M. Mutharia, L. Chan, R.P. Darveau, D.P. Speert, and G.B. Pier. 1983. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide 0 side-chains. Infect. Immun. 42:170-177.

34.Hancock, R.E.W., K. Poole, M. Gimple and R. Benz. 1983. Modification of the conductance, selectivity and concentration-dependent saturation of Pseudomonas aeruginosa protein P channels by chemical acetylation. Biochim. Biophys. Acta, 735:137-144.

35.Whitfield, C., R.E.W. Hancock, and J.W. Costerton. 1983. Outer membrane protein K of Escherichia coli: Purification and pore-forming properties in lipid bilayer membranes. J. Bacteriol. 156:873-879.

36.Darveau, R.P., S. MacIntyre, J.T. Buckley, and R.E.W. Hancock. 1983. Purification and reconstitution in lipid bilayer membranes of an outer membrane pore-forming protein of Aeromonas salmonicida. J. Bacteriol. 156:1006-1011.

37.Darveau, R.P., W.T. Charnetzky, R.E. Hurlbert, and R.E.W. Hancock. 1983. Effects of growth temperature, 47-megadalton plasmid, and calcium deficiency on the outer membrane protein porin and lipopolysaccharide composition of Yersina pestis EV76. Infect. Immun. 42:1092-1101.

38.Lam, J.S., L.M. Mutharia, R.E.W. Hancock, N. Hoiby, K. Lam, L. Baek, and J.W. Costerton. 1983. Immunogenicity of Pseudomonas aeruginosa outer membrane antigens examined by crossed immunoelectrophoresis. Infect. Immun. 42:88-98.

39.Downum, K.R., R.E.W. Hancock and G.H.N. Towers. 1983. Photodynamic action on Escherichia coli of natural acetylenic thiophenes, particularly 5-(buten-l-ynyl)-2,2'-bithienyl. Photobiochem. Photobiophys. 6:145-152.

40.Hancock, R.E.W. 1984. Alterations in outer membrane permeability. Annu. Rev. Microbiol. 38:237-264.

41.Benz, R., K. Poole, and R.E.W. Hancock. 1984. Characterization and chemical modification of small anion specific channels formed in lipid bilayer membranes by outer membrane protein P of Pseudomonas aeruginosa. Biophys. J. 45:81-82.

42.Hancock, R.E.W., E. Mouat, and D.P. Speert. 1984. Quantitation and identification of antibodies to the outer-membrane proteins of Pseudomonas aeruginosa in sera of patients with cystic fibrosis. J. Infect. Dis. 149:220-226.

43.Benz, R., R.P. Darveau, and R.E.W. Hancock. 1984. Outer-membrane protein PhoE from Escherichia coli forms anion-selective pores in lipid-bilayer membranes. Eur. J. Biochem. 140:319-324.

44. Darveau, R.P., R.E.W. Hancock and R. Benz. 1984. Chemical modification of the anion selectivity of the PhoE porin from the Escherichia coli outer membrane. Biochim. Biophys. Acta 774:69-74.

45.Mutharia, L.M., G. Crockford, W.C. Bogard, and R.E.W. Hancock. 1984. Monoclonal antibodies specific for Escherichia coli J-5 lipopolysaccharide: Cross-reaction with other gram-negative bacterial species. Infect. Immun. 45:631-636.

46.Loh, B., C. Grant and R.E.W. Hancock. 1984. Use of the fluorescent probe 1-N-phenylnapthylamine to study the interactions of aminoglycoside antibiotics with the outer membrane of Pseudomonas aeruginosa. Antimicrobiol. Agents Chemother. 26:546-551.

47.Hancock, R.E.W., and P.G.W. Wong. 1984. Compounds which increase the permeability of the Pseudomonas aeruginosa outer membrane. Antimicrob. Agents Chemother. 26:48-52.

48.Poole, K. and R.E.W. Hancock. 1984. Phosphate transport in Pseudomonas aeruginosa: Involvement of a periplasmic phosphate-binding protein. Eur. J. Biochem., 144:607-612.

49.Moore, R.A., L. Chan, and R.E.W. Hancock. 1984. Evidence for two distinct mechanisms of resistance to polymyxin B in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 26:539-545.

50.Hindahl, M.S., G.W.K. Crockford, and R.E.W. Hancock. 1984. Outer membrane protein NmpC of Escherichia coli: Pore-forming properties in black lipid bilayers. J. Bacteriol. 159:1053-1055.

51-100    

51.Mutharia, L.M., and R.E.W. Hancock. 1985. Characterization of two surface-localized antigenic sites on porin protein F of Pseudomonas aeruginosa. Canad. J. Microbiol. 31:381-386.

52.Benz, R., A. Schmidt, and R.E.W. Hancock. 1985. Ion selectivity of gram-negative bacterial porins. J. Bacteriol. 162:722-727.

53.Hancock, R.E.W., L.M. Mutharia and E.C.A. Mouat. 1985. Immunotherapeutic potential of monoclonal antibodies against Pseudomonas aeruginosa protein F. Europ. J. Clin. Microbiol. 4: 224-227.

54.Mutharia, L.M., and R.E.W. Hancock. 1985. Monoclonal antibody for an outer membrane lipoprotein of the Pseudomonas fluorescens group of the family Pseudomonadaceae. Int. J. Systemat. Bacteriol. 35:530-532.

55.Peterson, A.A., R.E.W. Hancock, and E.J. McGroarty. 1985. Binding of polycationic antibiotics and polyamines to lipopolysaccharides of Pseudomonas aeruginosa. J. Bacteriol. 164: 1256-1261.

56.Comeau, Y., J.J. Hall, R.E.W. Hancock, and W.K. Oldham. 1986. Biochemical model for enhanced biological phosphorous removal. Water Res. 20:1511-1521.

57.Moore, R.A., N.C. Bates and R.E.W. Hancock. 1986. Interaction of polycationic antibiotics with Pseudomonas aeruginosa lipopolysaccharide and lipid A studied by using dansyl-polymyxin. Antimicrob. Agents Chemother. 29: 496-500.

58.Parr, T.R., K. Poole, G.W.K. Crockford, and R.E.W. Hancock. 1986. Lipopolysaccharide-free Escherichia coli Omp F and Pseudomonas aeruginosa protein P porins are functionally active in lipid bilayer membranes. J. Bacteriol. 165: 523-526.

59.Hancock, R.E.W., and R. Benz. 1986. Demonstration and chemical modification of a specific phosphate binding site in the phosphate-starvation-inducible outer membrane porin protein P of Pseudomonas aeruginosa. Biochim. Biophys Acta 860: 699-707.

60.Poole, K., and R.E.W. Hancock. 1986. Isolation of a Tn501 insertion mutant lacking porin protein P of Pseudomonas aeruginosa. Molec. Gen. Genetics 202:403-409.

61.Poole, K., and R.E.W. Hancock. 1986. Phosphate-starvation-induced outer membrane proteins of the families Enterobacteriaceae and Pseudomonadaceae: demonstration of immunological cross-reactivity with an antiserum specific for porin protein P of Pseudomonas aeruginosa. J. Bacteriol. 165: 987-993.

62.Armstrong, S.K., T.R. Parr, C.D. Parker, and R.E.W. Hancock. 1986. Bordetella pertussis major outer membrane porin protein forms small, anion-selective channels in lipid bilayer membranes. J. Bacteriol. 166: 212-216.

63.Woodruff, W.A., T.R. Parr, R.E.W. Hancock, L. Hanne, T.I. Nicas, and B. Iglewski. 1986. Expression in Escherichia coli and function of Pseudomonas aeruginosa outer membrane porin protein F. J. Bacteriol. 167: 473-479.

64.Hancock, R.E.W., A. Schmidt, K. Bauer, and R. Benz. 1986. Role of lysines in ion selectivity of bacterial outer membrane porins. Biochim. Biophys. Acta 860: 263-267.

65.Huyer, M., T.R. Parr, R.E.W. Hancock and W.J. Page. 1986. Outer membrane porin protein of Campylobacter jejuni. FEMS Microbiol Lett. 37: 247-250.

66.Hancock, R.E.W. 1986. Intrinsic antibiotic resistance of Pseudomonas aeruginosa. J. Antimicrob. Chemother. 18: 653-659.

67.Moore, R.A., and R.E.W. Hancock. 1986. Involvement of outer membrane of Pseudomonas cepacia in aminoglycoside and polymyxin resistance. Antimicrob. Agents Chemother. 30: 923-926.

68.Kropinski, A.M., T.R. Parr, B. Angus, R.E.W. Hancock, W.C. Ghiorse, and E.P. Greenberg. 1987. Isolation of the outer membrane and characterization of the major outer membrane protein of Spirocheata aurantia. J. Bacteriol. 169:172-179.

69.Benz, R., and R.E.W. Hancock. 1987. Mechanism of ion transport through the anion-selective channel of the Pseudomonas aeruginosa outer membrane. J. Gen. Physiol. 89: 275-295.

70.Poole, K., T.R. Parr and R.E.W. Hancock. 1987. Phosphate-selective porins from the outer membranes of fluorescent Pseudomonas sp. Canad. J. Microbiol. 33: 63-69.

71.Parr, T.R., R.A. Moore, L.V. Moore and R.E.W. Hancock. 1987. Role of porins in intrinsic antibiotic resistance of Pseudomonas cepacia. Antimicrob. Agents Chemother. 31: 121-123.

72. Bayer, A.S., J. Peters, T.R. Parr, L. Chan and R.E.W. Hancock. 1987. Role of β-lactamase in in vivo development of ceftazidime resistance in experimental Pseudomonas aeruginosa endocarditis. Antimicrob. Agents. Chemother. 31: 253-258.

73.Hancock, R.E.W. 1987. Role of porins in outer membrane permeability. J. Bacteriol. 169: 929-933.

74.Speert, D.P., J.E. Dimmick, G.B. Pier, J.M. Saunders, R.E.W. Hancock and N.M. Kelly. 1987. An immunohistological evaluation of Pseudomonas aeruginosa pulmonary infection in two patients with cystic fibrosis. Pediatr. Res., 22:743-747.

75.Battershill, J., D.P. Speert, and R.E.W. Hancock. 1987. Use of monoclonal antibodies to protein F of Pseudomonas aeruginosa as opsonins for phagocytosis by macrophages. Infect. Immun. 55:2531-2533.

76.Kelly, N.M., J.P. Arbuthnott, J. Battershill, S. Kuo, and R.E.W. Hancock. 1987. Colonial dissociation and susceptibility to phagocytosis of Pseudomonas aeruginosa grown in a chamber implant model in mice. Infect. Immun., 55:2841-2843.

77.Angus, B.L., J. Fyfe and R.E.W. Hancock. 1987. The hydrophobic uptake pathway across the outer membrane of the antibiotic supersusceptible Pseudomonas aeruginosa mutant Z61. FEMS Microbiol. Lett., 48:153-57.

78.Angus, B.L., J. Fyfe and R.E.W. Hancock. 1987. Mapping and characterization of two mutations to antibiotic supersusceptibility in Pseudomonas aeruginosa. J. Gen. Microbiol., 133:2905-2914.

79.Lam, J.S., M.Y.C. Lam, L.A. MacDonald and R.E.W. Hancock. 1987. Visualization of Pseudomonas aeruginosa 0 antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin M in monoclonal antibodies. J. Bacteriol., 169:3531-3538.

80.Sawyer, J.G., N.L. Martin and R.E.W. Hancock. 1988. Interaction of macrophage cationic proteins with the outer membrane of Pseudomonas aeruginosa. Infect. Immun. 56:693-698.

81.Worobec, B., R. Siehnel, P. Gladman and R.E.W. Hancock. 1988. Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein. FEMS Microbiol. Lett. 52:235-238.

82.Worobec, B., N.L. Martin, W. McCubbin, C. Kay, G. Brayer and R.E.W. Hancock. 1988. Large-scale purification and biochemical characterization of crystallization-grade porin protein P from Pseudomonas aeruginosa. Biochim. Biophys. Acta 939:366-374.

83.Woodruff, W.A. and R.E.W. Hancock. 1988. Construction and characterization of Pseudomonas aeruginosa protein F-deficient mutants after in vitro and in vivo insertion mutagenesis of the cloned gene. J. Bacteriol. 170:2592-2598.

84.Siehnel, R., E.A. Worobec, and R.E.W. Hancock. 1988. Cloning of the Pseudomonas aeruginosa outer membrane porin protein P gene: Evidence for a linked region of DNA homology. J. Bacteriol. 170:2312-2318.

85.Rivera, M., L.E. Bryan, R.E.W. Hancock and E.J. McGroarty. 1988. Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa. Analysis of lipopolysaccharide chain length. J. Bacteriol. 170:512-521.

86.Rivera, M., R.E.W. Hancock, J.G. Sawyer, A. Haug and E.J. McGroarty. 1988. Enhanced binding of polycationic antibiotics to lipopolysaccharide from an aminoglycoside-supersusceptible, tolA mutant strain of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 32:649-655.

87.Moon, M.M., L.D. Hazlett, R.E.W. Hancock, R.S. Berk and R. Barrett. 1988. Monoclonal antibodies provide protection against ocular Pseudomonas aeruginosa infection. Invest. Opthamol. Vis. Sci. 29:1277-1284.

88.Siehnel, R.J., E. Worobec, and R.E.W. Hancock. 1988. Regulation of components of the Pseudomonas aeruginosa phosphate-starvation-inducible regulon in Escherichia coli. Molec. Microbiol. 2:347-352.

89.Hancock, R.E.W. and L. Chan. 1988. Outer membranes of environmental isolates of Pseudomonas aeruginosa. J. Clin. Microbiol. 26:2423-2424.

90.Hancock, R.E.W. and A. Bell. 1988. Antibiotic uptake into Gram-negative bacteria. Europ. J. Clin. Microbiol. Infect. Dis. 7:713-720.

91.Kelly, N.M., A. Bell and R.E.W. Hancock. 1989. Surface characteristics of Pseudomonas aeruginosa grown in a chamber implant model in mice and rats. Infect. Immun. 57:344-350.

92.Kluftinger, J., F. Lutz and R.E.W. Hancock. 1989. Pseudomonas aeruginosa cytotoxin: Periplasmic localization and inhibition of macrophages. Infect. Immun. 57:882-886.

93.Kluftinger, J.L., N.M. Kelly and R.E.W. Hancock. 1989. Stimulation by fibronectin of macrophage-mediated phagocytosis of Pseudomonas aeruginosa. Infect. Immun. 57:817-822.

94.Bell, A., and R.E.W. Hancock. 1989. Outer membrane protein H1 of Pseudomonas aeruginosa: Purification of the protein and cloning and nucleotide sequence of the gene. J. Bacteriol. 171:3211-3217.

95.Woodruff, W.A., and R.E.W. Hancock. 1989. Pseudomonas aeruginosa outer membrane protein F: Structural role and relationship to the Escherichia coli OmpA protein. J. Bacteriol. 171:3304-3309.

96.Lee, H.S., R.E.W. Hancock, and J.L. Ingraham. 1989. Properties of a Pseudomonas stutzeri outer membrane channel-forming protein (NosA) required for production of copper-containing N20 reductase. J. Bacteriol. 171:2096-2100.

97.Kelly, N.M., E.G. Rawling and R.E.W. Hancock. 1989. Determinants of the efficacy of tobramycin therapy against isogenic nonmucoid and mucoid derivatives of Pseudomonas aeruginosa PA01 growing in peritoneal chambers in mice. Antimicrob. Agents Chemother. 33:1207-1211.

98.Kluftinger, J.L., N.M. Kelly, B.H. Jost and R.E.W.Hancock. 1989. Fibronectin as an enhancer of nonopsonic phagocytosis of Pseudomonas aeruginosa by macrophages. Infect. Immun. 57:2782-2785.

99.Kelly, N.M., J.L. Kluftinger, B.L. Pasloske, W. Paranchych, and R.E.W. Hancock. 1989. Pseudomonas aeruginosa pili as ligands for nonopsonic phagocytosis by fibronectin-stimulated macrophages. Infect. Immun. 57:3841-3845.

100.Kelly, N.M., M.H. MacDonald, N. Martin, T.I. Nicas, and R.E.W. Hancock. 1990. Comparison of the outer membrane protein and lipopolysaccharide profiles of mucoid and nonmucoid Pseudomonas aeruginosa . J. Clin. Microbiol. 28:2017-2021.

101-150    
             

101.Siehnel, R.J., N.L. Martin, and R.E.W. Hancock. 1990. Sequence and relatedness in other bacteria of the Pseudomonas aeruginosa oprP gene coding for the phosphate-specific porin P. Molec. Microbiol. 4:831-838.

102.Hancock, R.E.W., R. Siehnel and N. Martin. 1990. Outer membrane proteins of Pseudomonas. Molec. Microbiol. 4:1069-1075.

103.Ullstrom, C.A., R. Siehnel, W. Woodruff, S. Steinbach, and R.E.W. Hancock. 1991. Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae: Sequence of the Pseudomonas syringae OprF gene. J. Bacteriol. 173:768-775

104.Hancock, R.E.W., S.W. Farmer, Z. Li, and K. Poole. 1991. Interaction of aminoglycosides with the outer membranes and purified lipopolysaccharide and OmpF porin of Escherichia coli. Antimicrob. Agents Chemother. 35:1309-1314.

105.Walker, S.G., R.E.W. Hancock, and J. Smit. 1991. Expression in Caulobacter cresentus of the phosphate-starvation-inducible porin OprP of Pseudomonas aeruginosa. FEMS Microbiol. Letters 77:217-222.

106.Bellido, F., J.-C. Pechere, and R.E.W. Hancock. 1991. Novel method for measurement of outer membrane permeability to new b-lactams in intact Enterobacter cloacae cells. Antimicrob. Agents Chemother. 35:68-72.

107.Bellido, F., J.-C. Pechere, and R.E.W. Hancock. 1991. Reevaluation of the factors involved in the efficacy of new b-lactams against Enterobacter cloacae. Antimicrob. Agents Chemother. 35:73-78.

108.Saravolac, E.G., N.F. Taylor, R. Benz, and R.E.W. Hancock. 1991. Purification of glucose-inducible outer membrane protein OprB of Pseudomonas putida and reconstitution of glucose-specific pores. J. Bacteriol. 173:4970-4976.

109.Bell, A., M. Bains, and R.E.W. Hancock. 1991. Pseudomonas aeruginosa outer membrane protein OprH: Expression from the cloned gene and function in EDTA and gentamicin resistance. J. Bacteriol. 173:6657-6664.

110.Hancock, R.E.W., and F. Bellido. 1992. Factors involved in the enhanced efficacy against Gram-negative bacteria of fourth generation cephalosporins. J. Antimicrob. Chemother. 29, Suppl. A:1-6.

111.Hancock, R.E.W., and F. Bellido. 1992. Antibiotic uptake: unusual results for unusual molecules. J. Antimicrob. Chemother. 29:235-243.

112.Farmer, S., Z. Li, and R.E.W. Hancock. 1992. Influence of outer membrane mutations on susceptibility of Escherichia coli to the dibasic macrolide azithromycin. J. Antimicrob. Chemother. 29:27-33.

113.Piddock, L.J.V., M.C. Hall, F. Bellido, M. Bains, and R.E.W. Hancock. 1992. A pleiotropic, post-therapy, enoxacin-resistant mutant of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 36:1057-1061.

114.Hancock, R.E.W., C. Egli, R. Benz, and R.J. Siehnel. 1992. Overexpression in Escherichia coli and functional analysis of a novel PPi selective porin, OprO, from Pseudomonas aeruginosa. J. Bacteriol. 174:471-476.

115.Siehnel, R.J., C. Egli, and R.E.W. Hancock. 1992. Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: Expression, purification and sequence. Molec. Microbiol.6:2319-2326.

116.Jaeger, K.E., F.-J. Adrian, H.E. Meyer, R.E.W. Hancock, and U.K. Winkler. 1992. Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein. Biochem. Biophys. Acta 1120:315-321.

117.Karunaratne, D.N., J.C. Richards and R.E.W. Hancock. 1992. Characterization of lipid A from Pseudomonas aeruginosa O-antigenic B-band lipopolysaccharide by 1D and 2D NMR and mass spectral analysis. Arch. Biochem. Biophys. 299:368-376.

118.Bellido, F., N.L. Martin, R.J. Siehnel, and R.E.W. Hancock. 1992. Reevaluation, using intact cells, of the exclusion limit and role of porin OprF in Pseudomonas aeruginosa outer membrane permeability. J. Bacteriol. 174:5196-5203.

119.Finnen, R.L., N.L. Martin, R.J. Siehnel, W.A. Woodruff, M. Rosok, and R.E.W. Hancock. 1992. Analysis of the Pseudomonas aeruginosa major outer membrane protein OprF by use of truncated OprF derivatives and monoclonal antibodies. J. Bacteriol. 174:4977-4985.

120.McCutcheon, A.R., S.M. Ellis, R.E.W. Hancock and G.H.N. Towers. 1992. Antibiotic screening of medicinal plants of the British Columbian native peoples. J. Ethnopharmacol. 37:213-223.

121.Kennel, W.L., C. Egli, R.E.W. Hancock, and S.C. Holt. 1992. Pore-forming ability of major outer membrane proteins from Wolinella recta ATCC 33238. Infect. Immun. 60:380-384.

122.Young, M., and R.E.W. Hancock. 1992. Fluoroquinolone supersusceptibility mediated by outer membrane protein OprH overexpression in Pseudomonas aeruginosa: Evidence for involvement of a non-porin pathway. Antimicrob. Agents Chemother. 36:2365-2369.

123.Young, M.L., M. Bains, A. Bell and R.E.W. Hancock. 1992. Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: Isolation of an OprH-deficient mutant by gene replacement techniques. Antimicrob. Agents Chemother. 36:2566-2568.

124.Huang, H., R.J. Siehnel, F. Bellido, E. Rawling, and R.E.W. Hancock. 1992. Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa. FEMS Microbiology Letters 97:267-274.

125.Hancock, R.E.W., and S.W. Farmer. 1993. Mechanism of uptake of deglucoteicoplanin amide derivatives across outer membranes of Escherichia coli and Pseudomonas aeruginosa. Antimicrob. Agents Chemother 37:453-456.

126.Benz, R., C. Egli, and R.E.W. Hancock. 1993. Anion transport through the phosphate-specific OprP-channel of the Pseudomonas aeruginosa outer membrane: Effects of phosphate, di- and tri-basic anions and of negatively-charged lipids. Biochim. Biophys. Acta, 1149: 224-230.

127.Egli, C., W.K. Leung, K.H. Müller, R.E.W. Hancock, and B.C. McBride. 1993. Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola. Infect. Immun. 61:1694-1699.

128.Mork, T., and R.E.W. Hancock. 1993. Mechanisms of nonopsonic phagocytosis of Pseudomonas aeruginosa. Infect. Immun. 61: 3287-3293.

129.Piers, K.L., M.H. Brown, and R.E.W. Hancock. 1993. Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria. Gene 134:7-13.

130.Wong, R.S.Y., H. Jost, and R.E.W. Hancock. 1993. Linker-insertion mutagenesis of Pseudomonas aeruginosa outer membrane protein OprF. Molec. Microbiol. 10:283-292.

131.Martin, N.M. E. G. Rawling, R. Wong, M. Rosok, and R.E.W. Hancock. 1993. Conservation of surface epitopes in Pseudomonas aeruginosa outer membrane porin protein OprF. FEMS Microbiol. Lett. 113:261-266.

132.Karunaratne, D.N., S. Farmer and R.E.W. Hancock. 1993. Synthesis of bulky b-lactams for inhibition of cell surface b-lactamase activity. Bioconjugate Chemistry. 4:434-439.

133.Huang, H., and R.E.W. Hancock. 1993. Genetic definition of the substrate selectivity of outer membrane porin protein OprD of Pseudomonas aeruginosa. J. Bacteriol. 175:7793-7800.

134.Piers, K., and R.E.W. Hancock. 1994. The interaction of a recombinant cecropin/melittin hybrid peptide with the outer membrane of Pseudomonas aeruginosa. Molec. Microbiol. 12:951-958.

135.Piers, K.L., M.H. Brown and R.E.W. Hancock. 1994. Improvement of outer membrane-permeabilizing and lipopolysaccharide-binding activities of an antimicrobial cationic peptide by C-terminal modification. Antimicrob. Agents Chemother. 38:2311-2316.

136.McCutcheon, A.R., S.M. Ellis, R.E.W. Hancock and G.H.N. Towers. 1994. Antifungal screening of medicinal plants of British Columbian native peoples. J. Ethnopharmacol. 44:157-169.

137.Blanco, D.R., K. Reimann, J. Skare, C.I. Champion, D. Foley, M.M. Exner, R.E.W. Hancock, J.N. Miller, and M.A. Lovett. 1994. Isolation of the outer membranes from Treponema pallidum and Treponema vincentii. J. Bacteriol. 176:6088-6099.

138.Saxena, G., A.R. McCutcheon, S. Farmer, G.H.N. Towers and R.E.W. Hancock. 1994. Antimicrobial constituents of Rhus glabra. J. Ethnopharmacol. 42:95-99.

139.Saxena, G., S. Farmer, R.E.W. Hancock, and G.H.N. Towers. 1995. Antimicrobial compounds from Alnus rubra. Intl. J. Pharmacognosy 33:33-36.

140.Saxena, G., S. Farmer, G.H.N. Towers, and R.E.W. Hancock. 1995. Use of specific dyes in the detection of antimicrobial compounds from crude plant extracts using a thin layer chromatography agar overlay technique. Phytochem. Anal. 6:125-129.

141.Rawling, E.G., N.L. Martin, and R.E.W. Hancock. 1995. Epitope mapping of the Pseudomonas aeruginosa major outer membrane porin protein OprF. Infect. Immun. 63:38-42.

142.Zhanel, G.G., J.A. Karlowsky, M.H. Saunders, R.J. Davidson, D.J. Hoban, R.E.W. Hancock, I. McLean, and L.E. Nicolle. 1995. Development of multiple-antibiotic-resistant (MAR) mutants of Pseudomonas aeruginosa after serial exposure to fluoroquinolones. Antimicrob. Agents Chemother. 39:489-495.

143.Exner, M.M., P. Doig, T.J. Trust and R.E.W. Hancock. 1995. Isolation and characterization of a family of porin proteins from Helicobacter pylori. Infect. Immun. 63:1567-1572.

144.Huang, H., D. Jeanteur, F. Pattus, and R.E.W. Hancock. 1995. Membrane topology and site-specific mutagenesis of Pseudomonas aeruginosa porin OprD. Molec. Microbiol. 16:931-941.

145.Shang, E.S., M.M. Exner, T.A. Summers, C. Martinich, C.I. Champion, R.E.W. Hancock, and D.A. Haake. 1995. The rare outer membrane protein, OmpL1, of pathogenic Leptospira species is a heat-modifiable porin. Infect. Immun. 63:3174-3181.

146.Blanco, D.R., C.I. Champion, M.E. Exner, H. Erdjument-Bromage, R.E.W. Hancock, P. Tempst, J.N. Miller, and M.A. Lovett. 1995. Porin activity and sequence analysis of a 31-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp1). J. Bacteriol. 177:3556-3562.

147.McCutcheon, A.R., T.E. Roberts, E. Gibbons, S.M. Ellis, L.A. Babiuk, R.E.W. Hancock, and G.H.N. Towers. 1995. Antiviral screening of British Columbian medicinal plants. J. Ethnopharmacol., 49:101-110.

148.Wong, R.S.Y., R.A. Wirtz, and R.E.W. Hancock. 1995. Pseudomonas aeruginosa outer membrane protein OprF as an expression vector for foreign epitopes: the effects of positioning and length on the antigenicity of the epitope. Gene, 158:55-60.

149.Liao, X., and R.E.W. Hancock. 1995. Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3. Antimicrob. Agents Chemother. 39:1871-1874.

151-200    
     

  150.Matsuura, H., G. Saxena, S.W. Farmer, R.E.W. Hancock and G.H.N. Towers. 1995. Antibacterial and antifungal compounds from Empetrum nigrum. Planta Medica, 61: 580.

151.Doig, P., M.M. Exner, R.E.W. Hancock, and T.J. Trust. 1995. Isolation and characterization of a conserved porin protein from Helicobacter pylori. J. Bacteriol. 177:5447-5452.

152.Forst, S., J. Waukau, G. Leisman, M. Exner and R.E.W. Hancock. 1995. Functional and regulatory analysis of the OmpF-like porin, OpnP, of the symbiotic bacterium Xenorhabdus nematophilus. Molec. Microbiol. 18:779-789.

153.Sukhan, A. and R.E.W. Hancock. 1995. Insertion mutagenesis of the Pseudomonas aeruginosa phosphate-specific porin OprP. J. Bacteriol. 177:4914-4920.

154.Lutwyche, P., M.M. Exner, R.E.W. Hancock and T.J. Trust. 1995. A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout. Infect. Immun. 63:3137-3142.

155.Matsuura, H., G. Saxena, S.W. Farmer, R.E.W. Hancock and G.H.N. Towers. 1996. An antibacterial thiophene from Balsamorhiza sagittata. Planta Medica. 62:65-66.

156.Liao, X., I. Charlebois, C. Ouellet, M.-J. Morency, K. Dewar, J. Lightfoot, J. Foster, R. Siehnel, H.Schweizer, J. Lam, R.E.W. Hancock and R.C. Levesque. 1996. Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome. Microbiology, 142: 79-86.

157.Saxena, G., S.W. Farmer, R.E.W. Hancock and G.H.N. Towers. 1996. Chlorochimaphilin: A new antibiotic from Moneses uniflora. J. Natural Products, 59:62-65.

158.Hancock, R.E.W. and F. Bellido. 1996. Antibacterial in vitro activity of fourth generation cephalosporins. J.Chemotherapy 8, Suppl. no. 2: 31-36.

159.Kondejewski, L.H., S.W. Farmer, D.S. Wishart, R.E.W. Hancock and R.S. Hodges. 1996. Gramicidin S is active against both gram-positive and gram-negative bacteria. Int. J. Pept. Prot. Res. 47:460-466.

160.Huang, H. and R.E.W. Hancock. 1996. The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginosa. J. Bacteriol., 178:3085-3090.

161.Matsuura, H., G. Saxena, S.W. Farmer, R.E.W. Hancock, and G.H.N. Towers. 1996. Antibacterial and antifungal polyine compounds from Glehnia littoralis ssp Leiocarpa. Planta Medica, 62:256-259.

162.Hancock, R.E.W. and T.J. Falla. 1996. Antimicrobial peptides: Broad-spectrum antibiotics from nature. Clin. Microbiol. Infect., 1:226-229.

163.Wong, R.S.Y. and R.E.W. Hancock. 1996. The effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier. FEMS Microbiol. Lett. 140:209-214.

164.Rehm, B.H.A. and R.E.W. Hancock. 1996. Membrane topology of the outer membrane protein OprH from Pseudomonas aeruginosa: PCR-mediated site-directed insertion and deletion mutagenesis. J. Bacteriol., 178:3346-3349.

165.Falla, T.J., D.N. Karunaratne, and R.E.W. Hancock. 1996. Mode of action of the antimicrobial peptide indolicidin. J. Biol. Chem., 271:19298-19303.

166.Sukhan A. and R.E.W. Hancock. 1996. The role of specific lysine residues in the passage of anions through the Pseudomonas aeruginosa porin OprP. J. Biol. Chem. 271:21239-21242.

167.Pumbwe, L., M.J. Everett, R.E.W. Hancock, and L.J.V. Piddock. 1996. Role of gyrA mutation and loss of OprF in the multiple antibiotic resistance (MAR) phenotype of Pseudomonas aeruginosa G49. FEMS Microbiol. Lett. 143: 25-28.

168.Gough, M., R.E.W. Hancock and N.M. Kelly. 1996. Anti-endotoxic potential of cationic peptide antimicrobials. Infect. Immun. 64: 4922-4927.

169.Kondejewski, L.H., S.W. Farmer, D.S. Wishart, C.M. Kay, R.E.W. Hancock, and R.S. Hodges. 1996. Effect of ring size of gramicidin S analogs on structure, antibacterial and hemolytic activity. J. Biol. Chem., 271: 25261-25268.

170.Blanco, D.R., C.I. Champion, M.M. Exner, E.S. Shang, J.T. Skare, R.E.W. Hancock, J.N. Miller and M.A. Lovett. 1996. Recombinant Treponema pallidum rare outer membrane protein 1 (Tromp1) expressed in Escherichia coli has porin activity and surface antigenic exposure. J. Bacteriol. 178: 6685-6692.

171.Mahasreshti, P.J., G.L. Murphy, J.H. Wyckoff, S. Farmer, R.E.W. Hancock and A.W. Confer. 1997. Purification and partial characterization of the OmpA family of proteins of Pasteurella haemolytica. Infect. Immun. 65: 211-218.

172.Hancock, R.E.W. 1997. The bacterial outer membrane as a drug barrier. Trends in Microbiol., 5: 37-42.

173.McCutcheon, A.R., R.W. Stokes, L.M. Thorson, S.M. Ellis, R.E.W. Hancock, and G.H.N. Towers. 1997. Anti-mycobacterial screening of British Columbian Medicinal Plants. Intl. Journ. Pharmacog. 35:77-83.

174.Bina, J.E., F. Nano and R.E.W. Hancock. 1997. Utilization of alkaline phosphatase fusions to identify secreted proteins, including potential efflux proteins and virulence factors from Helicobacter pylori. FEMS Microbiol. Lett. 148: 63-68.

175.Hancock, R.E.W. 1997. Peptide antibiotics. The Lancet, 349: 418-422.

176.Liao, J. and R.E.W. Hancock. 1997. Susceptibility to b-lactam antibiotics of Pseudomonas aeruginosa overproducing penicillin-binding protein 3. Antimicrob Agents Chemother. 41: 1158-1161.

177.Falla, T.J. and R.E.W. Hancock. 1997. Improved activity of a synthetic indolicidin analog. Antimicrob. Agents Chemother. 41: 771-775.

178.Hancock, R.E.W. 1997. Antibacterial Peptides and the outer membranes of Gram negative bacilli. J. Med. Microbiol. 46: 1-3.

179.Liao, X. and R.E.W. Hancock. 1997. Identification of a penicillin-binding protein 3 homologue, PBPC, in Pseudomonas aeruginosa: Gene cloning and growth phase dependent expression. J. Bacteriol. 179: 1490-6.

180.Champion, C.I., D.R. Blanco, M.M. Exner, H. Erdjument-Bromage, R.E.W. Hancock, P. Tempst, J.N. Miller and M.A. Lovett. 1997. Sequence analysis and recombinant expression of a 28-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp2). J. Bacteriol. 179: 1230-1238.

181.Wong, K.K.Y., K.Poole, N.Gotoh, and R.E.W.Hancock. 1997. Influence of OprM expression on multiple antibiotic resistance in Pseudomonas aeruginosa. Antimicrob.Agents Chemother. 41: 2009-2012.

182.Fidai, S., S. W. Farmer, and R.E.W. Hancock. 1997. Interaction of cationic peptides with bacterial membranes. Meth. Molec. Biol., 78: 187- 204.

183.Kobaisy, M., Z.Abramowski, L.Lerner, G.Saxena, R.E.W.Hancock, G.H.N.Towers, D.Doxsee, and R.W.Stokes. 1997. Antimycobacterial polyynes of Devil’sClub (Oplopanax horridus), a north american native medicinal plant. J. Natural Prod. 60: 1210-1213.

184.Luo, Y., J.R. Glisson, M.W. Jackwood, R.E.W. Hancock, M. Bains, I.N. Cheng, and C. Wang. 1997. Cloning and characterization of the major outer membrane protein gene (OmpH) of Pasteurella multocida X-73. J. Bacteriol. 179: 7856-7864.

185.Hancock, R.E.W., and R. Lehrer. 1998. Cationic peptides: a new source of antibiotics. Trends in Biotechnol. 16: 82-88. (includes cover photo)

186.Davey, M.L., R.E.W.Hancock, and L.M. Mutharia. 1998. Influence of culture conditions on expression of the 40-kilodalton porin protein of Vibrio anguillarum serotype 02. Appl. Environ. Microbiol. 64: 138-146.

187.Hancock, R.E.W. 1998. The therapeutic potential of cationic peptides. Expert Opinion Invest. Dis. 7: 167-174.

188.Hancock, R.E.W., R.Alm, J.Bina, and T.Trust. 1998. Helicobacter pylori: A surprisingly conserved bacterium. Nature Biotechnology 16: 216-217.

189.Houston,M.E., L.H.Kondejewski, D.N.Karunaratne, M.Gough, S.Fidai, R.S.Hodges, and R.E.W.Hancock. 1998. Influence of preformed α-helix and α-helix induction on the activity of cationic antimicrobial peptides. J.Peptide Res.52:81-88.

190.Rawling, E.G., F.S.L. Brinkman, and R.E.W. Hancock. 1998. Role of the carboxy-terminal half of Pseudomonas aeruginosa major outer membrane protein OprF in cell shape, growth in low-osmolarity medium, and peptidoglycan association. J. Bacteriol.180:3556-3562.

191.Zhang, L., T.Falla, M.Wu, S.Fidai, J.Burian, W.Kay, and R.E.W.Hancock. 1998. Determinants of recombinant production of antimicrobial cationic peptides and creation of peptide variants in bacteria. Biochem. Biophys. Res. Commun.247:674-680.

192.Dykes, G.A., R.E.W.Hancock, and J.W.Hastings. 1998. Structural variations in nisin associated with different membrane mimicking and pH environments. Biochem. Biophys. Res. Commun.247:723-727.

193.Wu, M., and R.E.W.Hancock. 1999. Interaction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane. J. Biol. Chem.274:29-35.

194.Hancock, R.E.W., and D.S.Chapple. 1999. Peptide antibiotics. Antimicrob. Agents Chemother. 43:1317-1323.

195.Gensberg, K., A.W.Smith, F.S.L.Brinkman, and R.E.W.Hancock. 1999. Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa. J. Antimicrob. Chemother. 43:607-608.

196.Wu, M., E.Maier, R.Benz, and R.E.W.Hancock. 1999. Mechanism of interaction of different classes of cationic antimicrobial peptides with planar bilayers and with the cytoplasmic membrane of Escherichia coli. Biochemistry 38:7235-7242.

197.Wu, M., and R.E.W.Hancock. 1999. Improved derivatives of bactenecin, a cyclic dodecameric antimicrobial cationic peptide. Antimicrob. Agents Chemother. 43:1274-1276.

198.Friedrich, C., M.G. Scott, N.Karunaratne, H.Yan, and R.E.W.Hancock. 1999. Salt-resistant alpha-helical cationic antimicrobial peptides. Antimicrob. Agents Chemother. 43:1542-1548.

199.Scott, M.G., H.Yan, and R.E.W.Hancock. 1999. Biological properties of structurally related a-helical cationic antimicrobial peptides. Infect. Immun. 67:2005-2009.

200.Zhang, L., R.Benz, and R.E.W.Hancock. 1999. Influence of proline residues on the antibacterial and synergistic activities of a-helical peptides. Biochemistry 38:8102-8111.

201-250    

201.Ochs, M.M., M.P.McCusker, M.Bains and R.E.W.Hancock. 1999. Negative regulation of the Pseudomonas aeruginosa outer membrane porin OprD selective for imipenem and basic amino acids. Antimicrob. Agents Chemother. 43:1085-1090.

202.Kondejewski, L.H., M.Jelokhani-Niaraki, S.W.Farmer, B. Lix, C.M.Kay, B.D.Sykes, R.E.W.Hancock, and R.S.Hodges. 1999. Dissociation of antimicrobial and hemolytic activities in cyclic peptide diastereomers by systematic alterations in amphipathicity. J. Biol. Chem. 274:13181-13192.

203.Brinkman, F.S.L., G. Schoofs, R.E.W.Hancock, and R.DeMot. 1999. Influence of a putative ECF sigma factor on expression of the major outer membrane protein, OprF, in Pseudomonas aeruginosa and Pseudomonas fluorescens. J.Bacteriol. 181:4746-4754.

204.Ochs, M.M., C.-D. Lu, R.E.W.Hancock and A.T. Abdelal. 1999. Amino acid-mediated induction of the basic amino acid-specific outer membrane porin OprD from Pseudomonas aeruginosa. J.Bacteriol. 181:5426-5432.

205.Macfarlane, E.L.A., A.Kwasnicka, M.M.Ochs, and R.E.W.Hancock. 1999. PhoP-PhoQ homologues in Pseudomonas aeruginosa regulate expression of the outer-membrane protein OprH and Polymyxin B resistance. Molec. Microbiol. 34:305-316.

206.Scott, M.G., M.R.Gold, and R.E.W.Hancock. 1999. Interaction of cationic peptides with lipoteichoic acid and Gram positive bacteria. Infect. Immun. 67:6445-6453.

207.Bina, J., R. A. Alm, M. Uria-Nuickelsen, S.R.Thomas, T.J.Trust, and R.E.W. Hancock. 2000. Helicobacter pylori uptake and efflux: Basis for intrinsic susceptibility to antibiotics in vitro. Antimicrob. Agents Chemother.44:248-254.

208.Scott, M.G..A.C.E.Vreugdenhil, W.A.Buurman, R.E.W.Hancock, and M.R.Gold. 2000. Cutting Edge: Cationic Antimicrobial Peptides Block the Binding of Lipopolysaccharide (LPS) to LPS Binding Protein. J. Immunol. 164: 549-553.

209.Bina, J., M.Bains, and R.E.W. Hancock. 2000. Functional expression in Escherichia coli and membrane topology of porin HopE, a member of a large family of conserved proteins in Helicobacter pylori. J.Bacteriol. 182:2370-2375.

210.Jia, X., A. Patrzykat, R. Devlin, P.A. Ackerman, G.K. Iwama, and R.E.W. Hancock. 2000. Antimicrobial peptides protect Coho salmon from Vibrio anguillarum infections. Appl. Environ Microbiol. 66:1928-1932.

211.Wong, K.K.Y., and R.E.W. Hancock. 2000. Insertion mutagenesis and membrane topology model of the Pseudomonas aeruginosa outer membrane protein OprM. J.Bacteriol. 182:2402-2410.

212.Chung, W., and R.E.W. Hancock. 2000. Action of lysozyme and nisin mixtures against lactic acid bacteria. Intl. J. Food Microbiol. 60:25-32.

213.Hancock, R.E.W., and M.G. Scott. 2000. The role of antimicrobial peptides in animal defences. Proc. Natl. Acad. Sci. USA. 97:8856-8861.

214.Johnstone, S.A., K. Gelmon, L.D. Mayer, R.E.W. Hancock, and M.B. Bally. 2000. In vitro characterization of the anticancer activity of membrane-active cationic peptides. I. Peptide-mediated cytotoxicity and peptide-enhanced cytotoxic activity of doxyrubicin against wild-type and P-glycoprotein over-expressing tumor cell lines. Anti-Cancer Drug Design. 15:151-160.

215.Rosenberger, C.M., M.G. Scott, M.R. Gold, R.E.W. Hancock, and B.B. Finlay. 2000. Salmonella typhimurium and lipopolysaccharide induce similar changes in macrophage gene expression. J. Immunol. 164:5894-5904.

216.Friedrich, C.L., D. Moyles, T.J.Beveridge, and R.E.W. Hancock. 2000. Antibacterial action of structurally diverse cationic peptides on Gram-positive bacteria. Antimicrob. Agents Chemother. 44:2086-2092.

217.Ochs, M.M., M.Bains, and R.E.W. Hancock. 2000. Role of putative loops 2 and 3 in imipenem passage through the specific porin OprD of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44:1983-1985.

218.Alm, R., J. Bina, B.M. Andrews, P. Doig, R.E.W. Hancock and T.J. Trust. 2000. Comparative genomics of Helicobacter pylori: Analysis of the outer membrane protein families. Infect. Immun. 68:4155-4168.

219.Hancock, R.E.W. 2000. Cationic antimicrobial peptides: towards clinical applications. Expert Opinion Invest. Dis. 9:1723-1729.

220.MacFarlane, E.L.A., A. Kwasnicka, and R.E.W.Hancock. 2000. Role of Pseudomonas aeruginosa PhoP-PhoQ in resistance to antimicrobial peptides and aminoglycosides. Microbiol. 146: 2543-2554.

221.Hancock, R.E.W., and D.P. Speert. 2000. Antibiotic resistance in Pseudomonas aeruginosa. Mechanisms and impact on treatment. Drug Resist. Updates. 3:247-255.

222.Stover, K.C., X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S.L. Brinkman, W. O. Hufnagle, D.J. Kowalik, M. Lagrou, R.L. Garber, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L.L. Brody, S.N. Coulter, K.R. Folger, A. Kas, R. Lim, K. Smith, D. Spencer, G.K.-S. Wong, Z. Wu, I. Paulsen, J. Reizer, M.H. Saier, R.E.W. Hancock, S. Lory, and M.V. Olson. 2000. Complete genome sequence of Pseudomonas aeruginosa: an opportunistic pathogen. Nature 406:959-964.

223.Brinkman, F.S.L., M.Bains and R.E.W.Hancock. 2000. The amino terminus of Pseudomonas aeruginosa outer membrane protein OprF forms channels in lipid bilayer membranes: correlation with a three-dimensional model. J. Bacteriol.182:5251-5255 (includes cover photo).

224.Brinkman, F.S.L., R.E.W. Hancock and K.C. Stover. 2000. Sequencing solutions: Use of volunteer annotators organized via the Internet. Nature 406:933.

225.Hancock, R.E.W. and G. Diamond. 2000. The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol. 8:402-410.

226.Osusky, M., G. Zhou, L.Osuska, R.E.W. Hancock, W.W. Kay and S. Misra. 2000. Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nature Biotech. 18:1162-1166.

227.Scott, M.G., C.M. Rosenberger, M.R. Gold, B.B. Finlay, and R.E.W. Hancock. 2000. An a-helical cationic antimicrobial peptide selectively modulates macrophage response to LPS and directly alters macrophage gene expression. J. Immunol. 165:3358-3365.

228.Jelokhani-Niaraki, M., L.H. Kondajewski, S.W.Farmer, R.E.W.Hancock, C.M.Kay, and R.S.Hodges. 2000. Diastereoisomeric analogues of gramicidin S: structure, biological activity and interaction with bilayers. Biochem. J. 349:747-755.

229.Scott, M.G., and R.E.W. Hancock. 2000. Cationic antimicrobial peptides and their multifunctional role in the immune system. Crit. Rev. Immunol. 20:407-431.

230.Zhang, L., P. Dhillon, H. Yan, S. Farmer, and R.E.W. Hancock. 2000. Interactions of bacterial cationic peptide antibiotics with outer and cytoplasmic membranes of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44:3317-3321.

231.Zhang, L., M.G. Scott, H. Yan, L.D. Mayer, and R.E.W.Hancock. 2000. Interaction of polyphemusin I and structural analogs with bacterial membranes, lipopolysaccharide and lipid monolayers. Biochem. 39:14504-14514.

232.Rozek, A., C.L. Friedrich, and R.E.W. Hancock. 2000. Structure of the bovine antimicrobial peptide indolicidin bound to dodecylphosphocholine and sodium dodecyl sulfate micelles. Biochem. 39:15765-15774.

233.Prosser, R.S., P.A. Luchette, P.W. Westermann, A. Rozek, and R.E.W. Hancock. 2000. Determination of membrane immersion depth with O2: A high pressure 19F NMR study. Biophys. J. 80:1406-1430.

234.Wong, K.K.Y., F.S.L. Brinkman, R.S. Benz, and R.E.W.Hancock. 2001. Evaluation of a structural model of Pseudomonas aeruginosa outer membrane protein OprM, an efflux component involved in intrinsic antibiotic resistance. J. Bacteriol. 183:367-374.

235.Brinkman, F.S.L., I. Wan, R.E.W. Hancock, A.M. Rose, and S.J. Jones. 2001. PhyloBLAST: A tool facilitating phylogenetic analysis of BLAST results. Bioinformatics 17:385-387.

236.Patrzykat, A., L. Zhang, V. Mendoza, G.K. Iwama, and R.E.W.Hancock. 2001. Synergy of histone-derived peptides of Coho salmon with lysozyme and the flounder peptide pleurocidin. Antimicrob. Agents Chemother. 45:1337-1342.

237.Yan, H., and R.E.W.Hancock. 2001. Synergistic interactions between mammalian antimicrobial defense peptides. Antimicrob. Agents Chemother. 45:1558-1560.

238.Friedrich, C.L., A.Rozek, A. Patrzykat, and R.E.W.Hancock. 2001. Structure and mechanism of action of an indolicidin peptide derivative with improved activity against Gram-positive bacteria. J. Biol. Chem. 276: 24015-24022.

239.Lange, C.F., R.E.W. Hancock, J. Samuel, and W.H. Finlay. 2001. In vitro aerosol delivery and regional airway surface liquid concentration of a liposomal cationic peptide. J. Pharmaceut. Sci. 90:1647-1657.

240.Hancock, R.E.W. 2001. Cationic peptides: effectors in innate immunity and novel antimicrobials. Lancet Infectious Diseases 1:156-164.

241.Brinkman, F.S.L., E.L.A. MacFarlane, P. Warrener, and R.E.W. Hancock. 2001. Evolutionary relationships amongst virulence associated histidine kinases. Infect. Immun. 69:5207-5211.

242.Zhang, L., A. Rozek, and R.E.W. Hancock. 2001. Interaction of cationic antimicrobial peptides with model membranes. J. Biol. Chem. 276:35714-35722.

243.Luchette, P.A., T.N. Vetman, R.S. Prosser, R.E.W.Hancock, M.P. Nieh, C.J. Glinka, S. Krueger, and J. Katsaras. 2001. Morphology of fast-tumbling bicelles: a small angle neutron scattering and NMR study. Biochim. Biophys. Acta 1513:83-94.

244.Chiu, C.H., S. Wong, R.E.W.Hancock, and D.P. Speert. 2001. Adherence of Burkholderia cepacia to respiratory tract epithelial cells and inhibition with dextrans. Microbiol. 147:2651-2658.

245.Hancock, R.E.W., and W.R. Strohl. 2001. Antimicrobials in the 21st Century. Curr. Opin. Microbiol. 4:491-492.

246. Hancock, R.E.W. 2001. A brief on bacterial biofilms. Nature Genetics 29:360. (News and views).

247.Desai, T.R., J.P. Wong, R.E.W.Hancock, and W.H. Finlay. 2002. A novel approach to the pulmonary delivery of liposomes in dry powder form to eliminate the deleterious effect of milling. J. Pharmaceut. Sci., 91:482-91.

248.Hancock, R.E.W., and A. Rozek. 2002. Role of membranes in the activities of antimicrobial cationic peptides. FEMS Microbiol. Lett, 206:143-149.

249.Hancock, R.E.W., and A. Patrzykat. 2002. Clinical development of cationic antimicrobial peptides: From natural to novel antibiotics. Curr. Drug Targets – Infect. Disorders. 2:79-83.

250.Patrzykat, A., C.L. Friedrich, L.Zhang, V. Mendoza, and R.E.W.Hancock. 2002. Sub-lethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli. Antimicrob. Agents Chemother. 46:605-614.

251-300    

251.Kondejewski, L.H., D.L. Lee, M. Jelokhani-Niaraki, S.W. Farmer, R.E.W. Hancock, and Robert S. Hodges. 2002. Optimization of microbial specificity in cyclic peptides by modulation of hydrophobicity within a defined structural framework. J. Biol. Chem. 277:67-74.

252.Hancock, R.E.W., and F.S.L. Brinkman. 2002. Function of Pseudomonas porins in uptake and efflux. Annu. Rev. Microbiol. 56:17-38.

253.Azghani, A., S. Idell, M.Bains, and R.E.W.Hancock. 2002. Pseudomonas aeruginosa outer membrane protein F is an adhesin in bacterial binding to lung epithelial cells in culture. Microb. Pathogenesis 33:109-114.

254.Gatto, N.T., S.M. Dabo, R.E.W. Hancock and A.W. Confer. 2002. Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multocida serotype A:3 (Omp28),. Vet. Microbiol. 87: 221-235.

255.Hirakata, Y., R. Srikumar, K. Poole, N. Gotoh, T. Suematsu, S. Kohno, S. Kamihira, R.E.W. Hancock, and D.P. Speert. 2002. Multidrug efflux systems play an important role in the invasiveness of Pseudomonas aeruginosa. J. Exp. Med. 196:109-118.

256.Roy, S., H.G. Lombart, W.D. Lubell, R.E.W. Hancock, and S.W. Farmer. 2002. Exploring relationships between mimic configuration, peptide conformation and biological activity in indolizidin-2-one amino acid analogs of gramicidin S. J. Pept. Res. 60:197-213.

257.Brinkman, F.S.L., J.L. Blanchard, A. Cherkasov, H. Greberg, Y. Av-Gay, R.C. Brunham, R.C. Fernandez, B.B. Finlay, S.P. Otto, B.F.F. Ouellette, P.J. Keeling, A.M. Rose, R.E.W. Hancock and S.J.M. Jones (2002). Evidence that plant-like genes in Chlamydia species reflect an ancestral relationship between Chlamydiaceae, cyanobacteria and the chloroplast. Genome Research. 12:1159-1167.

258.Scott, M.G., D.J. Davidson, M.R. Gold, D. Bowdish, and R.E.W. Hancock. 2002. The human antimicrobial peptide, LL-37, is a multifunctional modulator of innate immune responses. J. Immunol. 169:3883-3891.

259.Yoon, S.S., R.F. Hennigan, G.M. Hilliard, U.A.Ochsner, K.Parvatiyar, M.C.Kamani, H.L.Allen, T.R.DeKievit, P.R. Gardner, U.Schwab, J.J.Rowe, B.H.Iglewski, T.R.McDermott, R.P.Mason, D.J.Wozniak, R.E.W. Hancock, M.R.Parsek, T.L.Noah, R.C.Boucher, and D.J.Hassett. 2002. Pseudomonas aeruginosa anaerobic respiration in biofilms: Relationships to cystic fibrosis pathogenesis. Developmental Cell. 3:593-603.

260.Lupp, C., R.E.W. Hancock, and E.G. Ruby. 2002. The Vibrio fischeri sapABCDF locus is required for normal growth, both in culture and in symbiosis. Arch. Microbiol. 179:59-65.

261.Desai, T.R., R.E.W. Hancock, and W.H. Finlay. 2002. A facile method of delivery of liposomes by nebulization. J. Controlled Release 84:69-78.

262.Jo,J.T.H., F.S.L.Brinkman, and R.E.W.Hancock. 2003.Aminoglycoside efflux in Pseudomonas aeruginosa: involvement of novel outer membrane proteins. Antimicrob. Agents Chemother. 47:1101-1111.

263.Tamber, S., and R.E.W. Hancock. 2003. On the mechanism of solute transport in Pseudomonas. Frontiers in Bioscience. 8:S472-S483.

264.Boehr, D.D., K. Draker, K. Koteva, M. Bains, R.E. Hancock, and G.D. Wright. 2003 Broad-spectrum peptide inhibitors of aminoglycoside resistance enzymes. Chem. Biol. 10:189-196.

265.Llamas, M.A., J.J. Rodriguez-Herva, R.E.W. Hancock, W. Bitter, J. Tommassen, and J.L. Ramos. 2003. Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane. J. Bacteriol. 185:4707-16.

266.McPhee, J.B., S. Lewenza and R.E.W.Hancock. 2003. Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa. Molec. Microbiol. 50:205-219.

267.Haines, L.R., R.E.W. Hancock and T.W. Pearson. 2003. Cationic antimicrobial peptide killing of African trypanosomes and Sodalis glossinidius, a bacterial symbiont of the insect vector of sleeping sickness. Vector Borne and Zoonotic Dis. 3:175-186.

268.Hancock, R.E.W. 2003. Concerns regarding resistance to self-proteins (commentary). Microbiol. 149,3343-3344.

269.Rozek A., J.P. Powers, C.L. Friedrich, R.E.W.Hancock. 2003. Structure-based design of an indolicidin peptide analogue with increased protease stability. Biochemistry. 42:14130-14138.

270.Halevy, R., A. Rozek, S. Kolusheva, R.E.W. Hancock, and R. Jelinek. 2003. Membrane binding and permeation by indolicidin analogs studied by a biomimetic lipid/polydiacetylene vesicle assay. Peptides 24:1753-1761.

271.Powers, J.-P.S., and R.E.W. Hancock. 2003. The relationship between peptide structure and antibacterial activity. Peptides 24:1681-1691.

272.Desai, T.R., R.E.W. Hancock, and W.H. Finlay. 2003. Delivery of liposomes in dry powder form: aerodynamic dispersion properties. Europ. J. Pharmaceut. Sci. 20:459-467.

273.Anderson, R.C., R.E.W. Hancock and P.-L. Yu. 2004. Antimicrobial activity and bacterial membrane interaction of ovine-derived cathelicidins. Antimicrob. Agents Chemother. 48:673-676.

274.Davidson, D.J., A.J. Currie, G. Reid, D.M.E. Bowdish, K. MacDonald, R. Ma, R.E.W. Hancock, and D.P. Speert. 2004. The cationic antimicrobial peptide LL-37 modulates dendritic cell development and DC-induced T cell polarisation. J. Immunol. 172:1146-1156.

275.Powers, J.P., A. Rozek, and R.E.W. Hancock. 2004. Structure-activity relationships for the β-hairpin cationic antimicrobial peptide polyphemusin I. Biochim. Biophys. Acta 1698:239-250.

276.Hokamp K., F.M. Roche, M. Acab, M.E. Rousseau, B. Kuo, D. Goode, D. Aeschliman, J. Bryan, L. Babiuk, R.E.W. Hancock, and F.S.L. Brinkman. 2004. ArrayPipe: a flexible processing pipeline for microarray data. Nucl. Acids Res. 32:W471-474.

277.Bowdish D.M.E., D.J. Davidson, D.P. Speert, and R.E.W. Hancock. 2004. The human cationic peptide LL-37 induces activation of the extracellular signal regulated kinase and p38 kinase pathways in primary human monocytes. J. Immunol. 172:3758-3765.

278.Lee, D.L., J.P.S.Powers, K.Pflegerl, M.L.Vasil, R.E.W.Hancock, and R.S.Hodges. 2004. Effects of single D-amino acid substitutions on disruption of b-sheet structure and hydrophobicity in cyclic 14-residue antimicrobial peptide analogs related to gramicidin S. J. Peptide Res. 63:69-84.

279.Finlay, B.B., and R.E.W.Hancock. 2004. Can innate immunity be enhanced to treat infections? Nature Microbiol. Rev. 2:497-504.

280.Chapple D.S., R. Hussain, C.L. Joannou, R.E.W. Hancock, E. Odell, R.W. Evans, and G.Siligardi. 2004. Structure and association of human lactoferrin peptides with Escherichia coli lipopolysaccharide.
Antimicrob. Agents Chemother. 48:2190-2198.

281.Roche, F.M. K. Hokamp, M. Acab, L.A. Babiuk, R.E.W. Hancock, and F.S.L. Brinkman. 2004. ProbeLynx: A tool for updating the association of microarray probes to genes. Nucl. Acids Res. 32:W457-459.

282.Jung, D., A. Rozek, M. Okon, and R.E.W. Hancock. 2004. Structural transitions as determinants of the calcium dependent antibiotic daptomycin. Chem. Biol. 11:949-957.

283.Osusky, M. L. Osuska, R. E. Hancock, W. W. Kay, and S. Misra. 2004. Transgenic potatoes expressing a novel cationic peptide are resistant to late blight and pink rot. Transgenic Research 13:181-190.

284.Prather, D.T., M.Bains, R.E.W. Hancock, M.J. Filiatrault, and A.A. Campagnari. 2004. Differential Expression of Porins OmpP2A and OmpP2B of Haemophilus ducreyi. Infect. Immun.72:6271-6278.

285.Bowdish, D.M.E., D.J. Davidson, and R.E.W. Hancock. 2005. A re-evaluation of the role of host defence peptides in mammalian immunity. Current Protein Pept. Sci. 6:35-51.

286.McPhee, J.B. M.G. Scott, and R.E.W. Hancock. 2005. Design of host defence peptides for antimicrobial and immunity enhancing activities. Combinat. Chem. High Throughput Screening 8:257-272.

287.Lau, Y.E., A. Rozek, M.G. Scott, D.L. Goosney, D.J. Davidson and R.E.W. Hancock. 2005. Interaction and cellular localization of the human host defence peptide, LL-37, with lung epithelial cells. Infect. Immun.73:583-591.

288.Winsor, G., R. Lo, S.J. Ho Sui, K.S.E. Ung, S. Huang, D. Cheng, W.-K. Ho
Ching, R.E.W. Hancock, and F.S.L. Brinkman. 2005. Pseudomonas aeruginosa Genome Database and PseudoCAP: Facilitating community-based, continually updated, genome annotation. Nucleic Acids Research. 33:D338-D343.

289.Bowdish, D.M.E., D.J. Davidson, Y.E. Lau, K. Lee, M.G. Scott, and R.E.W. Hancock. 2005. Impact of cationic host defence peptides on anti-infective immunity. J. Leukocyte Biol. 77:451-459. (includes cover)

290.Lewenza, S., J.L. Gardy, F.S.L. Brinkman and R.E.W. Hancock. 2005. Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Genome Res. 15:321-329.

291.Hancock, R.E.W. 2005. Mechanisms of action of newer antibiotics for Gram positive bacteria. Lancet Infect. Dis. 5: 209-218.

292.Bowdish, D.M.E., D.J. Davidson, M.G. Scott, and R.E.W. Hancock. 2005. Immunomodulatory Activities of Small Host Defence Peptides. Antimicrob. Agents Chemother. in press. 49: 1727

293.Lewenza S., R.K. Falsafi, G. Winsor, W.J. Gooderham, J.B. McPhee, F.S.L. Brinkman and R.E.W. Hancock. 2005. Construction of a mini-Tn5-luxCDABE mutant library in Pseudomonas aeruginosa PAO1: a tool for identifying differentially regulated genes. Genome Res., in press. 15: 583

294.Bowdish, D.M.E., and R.E.W. Hancock. 2005. Anti-endotoxin properties of cationic host defence peptides and proteins. J. Endotox. Res. in press. 11: 230

295.Chen Y., C.T. Mant, S.W..Farmer, R.E.W. Hancock, M.L. Vasil, and R.S. Hodges. 2005. Rational design of a-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index.
J. Biol. Chem. 280:12316-29.

296.Zhang, L., J. Parente, S.M. Harris, D.E.Woods, R.E.W. Hancock, and T.J. Falla. 2005. Antimicrobial peptide therapeutics for cystic fibrosis. Antimicrob. Agents Chemother., in press. 49: 2921

297.Brazas, M., and R.E.W. Hancock. 2005. Using microarray gene signatures to elucidate mechanisms of antibiotic action and resistance. Drug Discovery Today 10:1245-1252.

298.McPhee, J.B., and R.E.W. Hancock. 2005. Function and therapeutic potential of host defence peptides. J. Peptide Sci. 11:677-687.

299.Brazas, M., and R.E.W. Hancock. 2005. Ciprofloxacin induction of a susceptibility determinant in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49:3222-3227.

300.Hilpert, K., R.Volkmer-Engert, T. Walter, and R.E.W. Hancock. 2005. High-throughput generation of small antibacterial peptides with improved activity. Nature Biotech. 23:1008-1012.

301-350    

301.Yevtushenko D.P, R. Romero, B.S. Forward, R.E. Hancock, W.W. Kay, and S. Misra. 2005. Pathogen-induced expression of a cecropin A-melittin antimicrobial peptide gene confers antifungal resistance in transgenic tobacco. J. Exptl. Botany 56:1685-1695.

302.Morck, D.W., S.P. Holland, H. Ceri, R. Hancock, M. Scott-Gough, V. Nguyen, E.J. Keith, and T.L. Lee. 2005. Use of polymyxin as an endotoxin blocker in the prevention of diffuse lamellar keratitis in an animal model. J. Refract. Surg. 21: 152-157.

303.Wu, L., O. Estrada, O. Zaborina, M. Bains, L. Shen, J.E. Kohler, N. Patel, M.W. Musch, E.B. Chang, Y. Fu, M.V. Olson, M.A. Jacobs, M.I. Nishimura, R.E.W. Hancock, J.R. Turner, and J.C. Alverdy. 2005. Recognition of host immune activation by Pseudomonas aeruginosa. Science 309:774-777.

304.Hancock, R.E.W., and J.B. McPhee. 2005. Salmonella’s sensor for host defense molecules. Cell 122:320-322.

305.Powers, J.P., A. Tan, A. Ramamoorthy, and R.E.W. Hancock. 2005. Solution structure and interaction of the antimicrobial polyphemusins with lipid membranes. Biochem. 44:15504-15513.

306.Tamber, S., and R.E.W. Hancock. 2006. The role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa. J. Bacteriol. 188:45-54.

307.Brown, K.L., and R.E.W. Hancock. 2006. Cationic host defence (antimicrobial) peptides. Curr. Opin. Immunol. 18:24-30.

308.Hancock, R.E.W., K.L. Brown, and N.Mookherjee. 2006. Host defence peptides from invertebrates – emerging antimicrobial strategies. Immunobiol. 211:315-322.

309.Mookherjee, N., K.L. Brown, D.M.E. Bowdish, S. Doria, R. Falsafi, K. Hokamp, F.M. Roche, R. Mu, G.H. Doho, J. Pistolic, J. Powers, J. Bryan, F.S.L. Brinkman and R.E.W. Hancock. 2006. Modulation of the Toll-like receptor-mediated inflammatory response by the endogenous human host defence peptide LL-37. J. Immunol. 176: 2455-2464.

310.Chen Y., A.I. Vasil, L. Rehaume, C.T. Mant, J.L. Burns, M.L. Vasil, R.E.W. Hancock, and R.S. Hodges. 2006. Comparison of biophysical and biologic properties of alpha-helical enantiomeric antimicrobial peptides. Chem. Biol. Drug Des. 67:162-173.

311.Lau, Y.E., D.M.E. Bowdish, C. Cosseau, R.E.W. Hancock and D.J. Davidson. 2006. Apoptosis of airway epithelial cells: human serum sensitive induction by the cathelicidin LL-37. Amer. J. Resp. Cell and Molec. Biol. 34:399-409.

312.Tamber, S., and R.E.W. Hancock. 2006. Involvement of two related porins, OprD and OpdP, in the uptake of arginine by Pseudomonas aeruginosa. FEMS Microbiol Lett. 260:23-29.

313.McPhee, J.B., M. Bains, G. Winsor, S. Lewenza, A. Kwasnicka, M.D. Brazas, F.S.L. Brinkman and R.E.W. Hancock. 2006. Contribution of the PhoP-PhoQ and PmrA-PmrB two-component regulatory systems to Mg2+-induced gene regulation in Pseudomonas aeruginosa. J. Bacteriol. 188:3995-4006.

314.Strauss, S.K. and R.E.W. Hancock. 2006. Mode of action of the new antibiotic for Gram-positive pathogens daptomycin: comparison with cationic antimicrobial peptides and lipopeptides. Biochim. Biophys. Acta Biomembranes 1758:1215-23.

315.Powers, J.P., M.M. Martin, D.L. Goosney and R.E.W. Hancock. 2006. The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment. Antimicrob. Agents Chemother. 50:1522-1524.

316.Barlow, P.G., Y. Li, T.S.Wilkinson, D.M.E. Bowdish, Y.E. Lau, C. Cosseau, C. Haslett, A.J. Simpson, R.E.W. Hancock, and D.J. Davidson. 2006. Human host defence peptide LL-37 modulates the induction of apoptotic pathways in primary cells of the innate immune system. J. Leuk. Biol. 80:509-520.

317.Marr, A.K., W.J. Gooderham, and R.E.W. Hancock. 2006. Antibacterial peptides for therapeutic use: obstacles and realistic outlook. Curr. Opin. Pharmacol. 6:468-472.

318.Jenssen, H., P. Hamill, and R.E.W. Hancock. 2006. Peptide antimicrobial agents. Clin. Microbiol. Rev. 19:491-511.

319.Hilpert, K., M.R. Elliott, R. Volkmer-Engert, P. Henklein, O. Donini, Q. Zhou, D.F.H. Winkler and R.E.W. Hancock. 2006. Sequence requirements and a novel optimization strategy for short antimicrobial peptides. Chem Biol. 13:1101-1107. (Paper is previewed in the same issue in Sahl, H.G. 2006. Optimizing antimicrobial host defense peptides. pp. 1015-1017).

320.Hancock, R.E.W., and H.G. Sahl. 2006. Antimicrobial and host-defence peptides as novel anti-infective therapeutic strategies. Nature Biotech. 24:1551-1557.

321.Davidson, D.J., A.J. Currie, DM.E. Bowdish, K.L. Brown, C.M. Rosenberger, RC. Ma, J. Bylund, P.A. Campsall, A. Puel, C. Picard, J.-L. Casanova, S.E. Turvey, R.E.W. Hancock, R.S. Devon, and D.P. Speert. 2006. IRAK-4 mutation (Q293X): rapid detection, and characterisation of defective posttranscriptional TLR/IL-1R responses in human myeloid and non-myeloid cells. J. Immunol. 177:8202-11.

322.Hancock, R.E.W. 2007. The end of an era? Nature Reviews Drug Discovery 6:28. (Foreword)

323.Mookherjee, N., H.L. Wilson, S. Doria, Y. Popowych, R. Falsafi, J. Yu, Y. Li, S. Veatch, F.M. Roche, K.L. Brown, F.S.L. Brinkman, K. Hokamp, A. Potter, L. A. Babiuk, P.J. Griebel and R.E.W. Hancock. 2007. Bovine and human cathelicidin cationic host defence peptides similarly suppress global transcriptional responses to bacterial lipopolysaccharide. J. Leuk. Biol. 80:1563-74.

324.Tamber, S., E. Maier, R. Benz, and R.E.W. Hancock. 2007. Characterization of OpdH, a Pseudomonas aeruginosa porin involved in the uptake of tricarboxylates. J. Bacteriol. 189:929-939.

325.Marr, A.K., J. Overhage, M. Bains and R.E.W. Hancock. 2007. The Lon protease of Pseudomonas aeruginosa is induced by aminoglycosides and is involved in biofilm formation and motility. Microbiol. 153:474-82.

326.Brown, K.L, N. Mookherjee, and R.E.W. Hancock. 2007. Antimicrobial, Host Defence Peptides and Proteins. Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester http://www.els.net/; DOI: 10.1002/9780470015902.a0001212.pub2.

327.Overhage, J., S. Lewenza, A.K. Marr and R.E.W. Hancock. 2007. Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant library. J. Bacteriol. 189:2164-2169.

328.Moraes, T.F., M. Bains, R.E.W. Hancock, and N.C. Strynadka. 2007. An arginine ladder mediates phosphate specific transfer across membranes. Nature Struct. Molec. Biol. 14:85-87.

329.Veatch, S.L., S.S.W. Leung, R.E.W. Hancock, J.L. Thewalt. 2007. Fluorescent probes alter miscibility phase boundaries in ternary vesicles. J. Phys. Chem. B 111:502-4.

330.Pan, Y.-L., J.T.J. Cheng, J. Hale, J. Pan, R.E.W. Hancock, and S.K. Straus. 2007. Characterization of the structure and membrane interaction of the antimicrobial peptides Aurein 2.2 and 2.3 from Australian Southern Bell Frog. Biophys. J. 92:2854-2864.

331.Bylund, J., K.L. MacDonald, K.L. Brown, P. Mydel, L.V. Collins, R.E.W. Hancock and D.P. Speert. 2007. Enhanced inflammatory responses of chronic granulomatous disease leukocytes involve reactive oxygen species-independent activation of NF-kB. Europ. J. Immunol. 37:1087-1096.

332.Barsky, A., J.L. Gardy, R.E.W. Hancock and T. Munzner. 2007. Cerebral: a Cytoscape plugin for layout of and interaction with biological networks using subcellular localization annotation. Bioinformatics 23:1040-1042.

333.Mookherjee, N., and R.E.W. Hancock. 2007. Cationic Host Defence Peptides: Innate immune regulatory peptides as a novel approach for treating infections. Cell. Molec. Life Sci. 64:922-933.

334.Scott, M.G., E. Dullaghan, N. Mookherjee, N. Glavas, M. Waldbrook, A. Thompson, A. Wang, K. Lee, S. Doria, P. Hamill, J. Yu, Y. Li, O. Donini, M.M. Guarna, B.B. Finlay, J.R. North, and R.E.W. Hancock. 2007. An anti-infective peptide that selectively modulates the innate immune response. Nature Biotech. 25: 465-472. Highlighted in various journals including Nature Reviews Drug Discovery 6, 347; May 2007.

335.Hilpert, K., D. Winkler, and R.E.W. Hancock. 2007. Peptide arrays on cellulose support: a time and cost efficient method for synthesizing large numbers of peptides in a parallel and addressable fashion. Nature Protocols 2:1333-1349.

336.Fjell, C.D., R.E.W. Hancock and A. Cherkasov AMPer. 2007. A database and an automated discovery tool for antimicrobial peptides. Bioinformatics 23:1148-1155.

337.Clements, A., D. Tull, A.W. Jenney, J.L. Farn, S.-H Kim, Bishop, R.E., J.B. McPhee, R.E.W Hancock, E.L. Hartland, M.J. Pearse, O.L.C. Wijburg, D.C. Jackson, M.J. McConville and R. A. Strugnell. 2007. Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides. J. Biol. Chem. 282:15569-15577.

338.Brown, K.L., C. Cosseau, J.L. Gardy and R.E.W. Hancock. 2006. Complexities of targeting innate immunity to treat infection. Trends Immunol. 28:260-266.

339.Hilpert, K., D. Winkler, and R.E.W. Hancock. 2007. Cellulose-bound peptide arrays: Preparation and applications. Biotechnol. Genet. Engineer. Rev. 24:31-106.

340.Hilpert, K., and R.E.W. Hancock. 2007. Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology. Nature Protocols 2:1652-1660.

341.Stewart-Ornstein, J., A.P. Hitchcock, D. Hernàndez-Cruz, P. Henklein, J. Overhage, K. Hilpert, J.D. Hale and R.E.W. Hancock. 2007. Using intrinsic X-ray absorption spectral differences to identify and map peptides and proteins. J. Phys. Chem. 111:7691-7699.

342.Mookherjee, N., L. Rehaume and R.E.W. Hancock. 2007. Cathelicidins and functional analogues as anti-sepsis molecules. Expert Opin. Therap. Targets 11:993-1004.

343.Jenssen H., T. Lejon, K. Hilpert, C. Fjell, A.Cherkasov and R.E.W.Hancock. 2007. Evaluating different descriptors for model design of antimicrobial 12-mer peptides with enhanced activity towards Pseudomonas aeruginosa. Chem. Biol. Drug Design 70:134-42.

344.Brazas, M.D., E.B.M. Breidenstein, J. Overhage and R.E.W.Hancock. 2007. Role of Lon protease in resistance of Pseudomonas aeruginosa to ciprofloxacin. Antimicrob. Agents Chemother. 51:4276-4283.

345.Hancock, R.E.W. 2007. The complexities of antibiotic action. Molec. Systems Biology. (News and Views). In press. 3 : 142

346.Yu, J., N. Mookherjee, K. Wee, D.M.E. Bowdish, J. Pistolic, Y. Li, L. Rehaume, and R.E.W. Hancock. 2007. Host defence peptide LL-37, in synergy with inflammatory mediator IL-1b, augments immune responses by multiple pathways. J. Immunol. 179:7684-7691.

347.Ho, S.W., D. Jung, J.R. Calhoun, J.D. Lear, M. Okon, W.R.P. Scott, R.E.W. Hancock, and S.K. Straus. 2007. Effect of divalent cations on the structure of the antibiotic daptomycin. Europ. J. Biophys., in press.

348.Hale, J.D.F. and R.E.W. Hancock. 2007. The alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Future Drugs, in press.

349.Scott W.R., S.B. Baek, D. Jung, R.E.W. Hancock and S.K. Straus. 2007. NMR structural studies of the antibiotic lipopeptide daptomycin in DHPC micelles. Biochim. Biophys. Acta 1768:3116-26.

350.Bhargava, A., M. Osusky, R.E.W. Hancock, B.S. Forward, W.M. Kay, and S. Misra. 2007. Antiviral indolicidin variant peptides: Evaluation for broad-spectrum disease resistance in transgenic Nicotiana tabacum. Plant Sci. 172:515-23.

351-438    

351.Bhargava, A., M. Osusky, B.S. Forward, R.E.W. Hancock, W.M. Kay, and S. Misra. 2007. Expression of a polyphemusin variant in transgenic tobacco confers resistance against plant pathogenic bacteria, fungi and a virus. Plant Cell Tiss. Organ Cult. 88:301-12.

352.Jenssen H., C. Fjell, A. Cherkasov and R.E.W. xHancock. 2008. QSAR modeling and computer-aided design of antimicrobial peptides. J. Pept. Sci. 14:110-114.

353.Wiegand, I., K. Hilpert, and R.E.W. Hancock. 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 3:163-175.

354.Goldberg, J.G., R.E.W. Hancock, R.E. Parales, J. Loper, and P. Cornelis. 2008. Pseudomonas 2007. J. Bacteriol. E-pub ahead of print PMID: 18165299

355.Overhage, J., M. Bains, M.D. Brazas, and R.E. W. Hancock. 2008. Swarming of Pseudomonas aeruginosa is a complex adaptation leading to increased production of virulence factors and antibiotic resistance. J. Bacteriol. PMID: 18245294. 190: 2671

356.Jiang, Z., A.I. Vasil, J. Hale, R.E.W. Hancock, M.L. Vasil and R.S. Hodges. 2007. Effects of net charge and the number of positively charged residues on the biological activity of amphipathic alpha-helical cationic antimicrobial peptides. Biopolymers. Epub ahead of print. PMID: 18098173. 90:369

357.Le Gac, M., M.D. Brazas, M. Bertrand, J.G. Tyerman, C.C. Spencer, R.E.W. Hancock, and M. Doebeli. 2008. Metabolic changes associated with adaptive diversification in Escherichia coli. Genetics 178:1049-1060.

358.Fjell, C.D., H. Jennsen, P. Fries, P. Aich, P. Griebel, K. Hilpert, R. E. W. Hancock, and A. Cherkasov. 2008. Identification of novel host defense peptides and the absence of alpha-defensins in the bovine genome. Proteins: Struct. Function Bioinformat. In press.73: 420

359.Blohmke, C.J., A.F. Hirschfeld, I. Elias, R.E. Victor, D.G. Hancock, A.G.F. Davidson, P.G. Wilcox, K.D. Smith, J. Overhage, R.E.W. Hancock, and S.E. Turvey. 2008. Innate immunity mediated by TLR5 as a novel anti-inflammatory target for cystic fibrosis lung disease. J. Immunol. 180:7765-7773.

360.Jenssen, H., K. Sandvik, J.H. Andersen, R.E.W. Hancock, and T.J. Gutteberg. 2008. Inhibition of HSV cell-to-cell spread by lactoferrin and lactoferricin. Antiviral Res. 79:192-198.

361.Jung, D., J.P. Powers, S.K. Straus, R.E.W. Hancock. 2008. Lipid-specific binding of the calcium-dependent antibiotic daptomycin leads to changes in lipid polymorphism of model membranes. Chem. Biophys. Lipids 154:120-128.

362.Rehaume, L., and R.E.W. Hancock. 2008. Neutrophil-derived defensins as modulators of innate immune function. Crit. Rev. Immunol. 28:185-200.

363.Brown, K.L., J. Bylund, K.L. MacDonald, G.X. Song-Zhao, M.R. Elliott, R. Falsafi, R.E.W.Hancock and D.P. Speert. 2008. ROS-deficient monocytes have aberrant gene expression that correlate with inflammatory disorders of chronic granulomatous disease. Clin Immunol. 129:90-102.

364.Gooderham W.J., M. Bains, J.B. McPhee, I. Wiegand, R.E.W. Hancock. 2008. Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation and swarming motility of PsrA in Pseudomonas aeruginosa. J. Bacteriol. 190:5624-5634. Highlighted in ASM News, October 2008.19:5624

365.Leung B.O., A.P. Hitchcock, J.L. Brash, A. Scholl, A. Doran, P. Henklein, J. Overhage, K.Hilpert, J.D. Hale, R.E.W. Hancock. 2008. X-ray spectromicroscopy study of competitive adsorption of protein and peptide onto polystyrene-poly (methyl methacrylate). Biointerphases 3:FB27-FB35.

366.Cosseau, C., D.A. Devine, E. Dullaghan, J.L. Gardy, A. Chikatamarla, S. Gellatly, L.L. Yu, J. Pistolic, R. Falsafi, J. Tagg, and R.E.W. Hancock. 2008. The commensal Streptococcus salivarius K12 down -regulates the innate immune responses of human epithelial cells and promotes host-microbe homeostasis. Infect.Immun.76:4163-4175. Selected as a Spotlight by the Journal and as a Journal highlight in the October 2008 issue of ASM News.76:4163

367.Lynn, D.J., G.L. Winsor, C. Chan, N. Richard, M.R. Laird, A. Barsky, J.L. Gardy, F.M.Roche, T.H.W. Chan, N. Shah, R. Lo, M. Naseer, J. Que, M. Yau, M. Acab, D. Tulpan, M.Whiteside, A. Chikatamarla, B. Mah, T.M. Munzner, K. Hokamp, R.E.W. Hancock, and F.S.L. Brinkman. 2008. Facilitating systems level analyses of the mammalian innate immune response. Molec. Systems Biol. 4:218. Epub.

368.Overhage, J., A. Campisano, M. Bains, E.C.W. Torfs, B.H.A. Rehm, and R.E.W. Hancock.2008. The human host defence peptide LL-37 prevents bacterial biofilm formation. Infect.Immun. 76:4176-4182. Recommended by the Faculty of 1000 Medicine.

369.Wiegand, I., A.K. Marr, E.B.M. Breidenstein, K.N. Schurek, P. Taylor, and R.E.W.Hancock. Mutator genes giving rise to decreased antibiotic susceptibility in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 52:3810-3813.

370.Hirche, T.O., R. Benabid, G. Deslee, S. Gangloff, S. Achilefu, M. Guenounou, F. Lebargy, R.E.W. Hancock, and A. Belaaouaj. 2008. Neutrophil elastase is required for innate host defense against Pseudomonas aeruginosa. J. Immunol. 181:4945-4954.

371.Breidenstein, E.B.M., B.K. Khaira, I. Wiegand and R.E.W. Hancock. 2008. A complex ciprofloxacin resistome revealed by screening of a library of Pseudomonas aeruginosa mutants for altered susceptibility. Antimicrob. Agents Chemother. 52:4486-4491.

372.Schurek, K.N., A.K. Marr, P.K. Taylor, I. Wiegand, L. Semenec, B. K. Khaira, and R.E.W.Hancock. 2008. Novel genetic determinants of low-level aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52:4213-4219.

373.Jiang Z., B.J. Kullberg, H. van der Lee, A.I. Vasil, J.D. Hale, C.T. Mant, R.E.W. Hancock, M.L. Vasil, M.G. Netea and R.S. Hodges. 2008. Effects of Hydrophobicity on the Antifungal Activity of -Helical Antimicrobial Peptides. Chem. Biology Drug Design 72:483-495.

374.Wasan K.M., S.J. Thornton, Ian Bell, R.E. Goulding, M. Gretes, A.P. Gray, R.E.W.Hancock and B. Campbell. 2009. The global access initiative at the University of British Columbia (UBC): Availability of UBC discoveries and technologies to the developing world (Invited Commentary) J. Pharmaceut. Sci. 89:791-794.

375.Hamill, P., K. Brown, H. Jenssen and R.E.W. Hancock. 2009. Novel anti-infectives: is host defence the answer? Curr. Opin. Biotechnol. 19:628-636.

376.Nijnik, A., and R.E.W. Hancock. 2009. The roles of cathelicidin LL-37 in immune defences and novel clinical applications. Curr. Opin. Haematol. 16:41-47.

377.Jenssen, H, and R.E.W. Hancock. 2009. Antimicrobial properties of lactoferrin. Biochimie. 91:19-29.

378.Winstanley, C., M.G.I. Langille, J.L. Fothergill, I. Kukavica-Ibrulj, C. Paradis-Bleau, F.Sanschagrin, N.R. Thomson, G.L. Winsor, M.A. Quail, N. Lennard, A. Bignell, L. Clarke,K. Seeger, D. Saunders, D. Harris, J. Parkhill, R.E.W. Hancock, F.S.L. Brinkman, and R.C.Levesque. 2009. Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool Epidemic Strain of Pseudomonas aeruginosa. Genome Res. 19:12-23. Discussed in Lancet Infect. Dis. 9:14, 2009.

379.Marr,A. K., H. Jenssen, M. R. Moniri, R.E.W. Hancock and N. Panté. 2009. Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of herpes simplex virus-1. Biochimie 91:160-164.

380.Nijnik, A., and R.E.W. Hancock. 2009. Host defence peptides – antimicrobial and immunomodulatory activity and potential applications for tackling antibiotic-resistant infections. Emerg. Health Threats J. 2:e1. Epub.

381.Cheng, J.T.J., J.D. Hale, M. Elliot, R.E.W. Hancock, and S.K. Straus. 2009. The effect of membrane composition on the antimicrobial peptides Aurein 2.2 and 2.3 from Australian southern bell frogs. Biophys. J. 96:552-565.

382.Winsor, G.L., T. Van Rossum, R. Lo, B. Khaira, MD. Whiteside, R.E.W. Hancock and F.S.L. Brinkman. 2009. Pseudomonas Genome Database: Facilitating user-friendly, comprehensive comparisons of microbial genomes. Nucl. Acids Res. 37 (Database issue):D483-488.

383.Cherkasov, A., K. Hilpert, H. Jenssen, C.D. Fjell, M. Waldbrook, S.C. Mullaly, R. Volkmer and R.E.W. Hancock. 2009. Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic resistant Superbugs. ACS Chemical Biol. 4:65-74.

384.Hilpert, K., M. Elliott, H. Jenssen, J. Kindrachuk, CD. Fjell, J. Körner, D.F.H. Winkler, L.L.Weaver, P. Henklein, A.S. Ulrich, S.H.Y. Chiang, S.W. Farmer, N. Pante, R. Volkmer, and R.E.W. Hancock. 2009. Screening and characterization of surface-tethered cationic peptides for antimicrobial activity. Chem. Biol. 16:58-69.

385.Pistolic, J., C. Cosseau, Y. Li, J.Yu, N.Filewod, S. Gellatly, L. Rehaume, D.M. Bowdish,and R.E.W. Hancock. 2009. Host defense peptide LL-37 induces IL-6 expression in human bronchial epithelial cells by activation of the NF-B signaling pathway. J. Innate Immun. 1:254–267.

386.Gooderham, W.J., and R.E.W. Hancock. 2009. Regulation of virulence and antibiotic resistance by two -component regulatory systems in Pseudomonas aeruginosa. FEMS Microbiol. Rev. 33:279-294.

387.Garlapati, S., M. Facci, M. Polewicz, S. Strom, L.A. Babiuk, G. Mutwiri, R.E.W. Hancock, M.R. Elliott, and V. Gerdts. 2009. Strategies to link innate and adaptive immunity when designing vaccine adjuvants. Vet. Immunol. Immunopathol. 128:184-91.

388.Gooderham, W.J., S. Gellatly, F. Sanschagrin, J.B. McPhee, M. Bains, Celine Cosseau, R.C. Levesque, and R.E.W. Hancock. 2009. The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa. Microbiology 155:699-711. Highlighted in the 'Hot off the Press' section of the May 2009 issue of the SGM quarterly magazine “Microbiology Today”.

389.Haines, L.R., J.M. Thomas, A.M. Jackson, B.A. Eyford, M. Razavi, C.N. Watson, B. Gowen, R.E.W. Hancock, and T.W. Pearson. 2009. Killing of Trypanosomatid parasites by a modified bovine host defense peptide, BMAP-18. PLoS Neglected Trop. Dis. 3:e373. Epub.

390.Mader, J.S., N. Joza, J.M. Penniger, N. Mookherjee, R.E.W. Hancock, and R.C. Bleackley. 2009 The human cathelicidin, LL-37, kills through activation of the mitochondrialassociated pathway of apoptosis in a caspase-independent, AIF-dependent manner. Molec. Cancer Res. 7:689-702.

391.Fjell, C.D., H. Jenssen, K. Hilpert, W.A. Cheung, N. Panté, R.E.W. Hancock and A.Cherkasov. 2009. Identification of Novel Antibacterial Peptides by Chemoinformatics and Machine Learning. J. Med Chem. 52:2006-2015.

392.Mookherjee, N., P. Hamill, J. Gardy, D. Blimkie, R. Falsafi, A. Chikatamarla, D.J. Arenillas, S. Doria, T.R. Kollmann, R.E.W. Hancock. 2009. Systems biology evaluation of immune responses induced by human host defence peptide LL-37 in mononuclear cells. Molec. BioSystems 5:483-496. Epub.

393.Kovacs-Nolan, J., L. Latimer, A. Landi, H. Jenssen, R.E.W. Hancock, L.A. Babiuk, and S. van Drunen Littel-van den Hurk. 2009. The novel adjuvant combination of CpG ODN, indolicidin and polyphosphazene induces potent antibody and cell-mediated immune responses in mice. Vaccine 27:2055-2064.

394.Gardy, J.L., D.J. Lynn, F.S.L. Brinkman, and R.E.W. Hancock. 2009. Enabling a systems biology approach to immunology: focus on innate immunity. Trends in Immunology, 30:249-262. Feature cover article.

395.Arafah, S., M.-L. Rosso1, L. Rehaume, R.E.W. Hancock, M. Simonet, and M. Marceau. 2009. An iron -starvation inducible LysR-type regulator regulates the polymyxin resistance pmrF operon and controls virulence in Yersinia pseudotuberculosis. Microbiology 155:2168-2181.

396.McPhee, J.B., S. Tamber, M. Bains, E. Maier, S. Gellatly, A. Lo, R. Benz, and R.E.W.Hancock. 2009. The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically-regulated, cation-selective channel. FEMS Microbiol. Lett. 296:241-247.

397.Filewod, N., J. Pistolic, R.E.W. Hancock. 2009. Low concentrations of LL-37 alter IL-8 production by keratinocytes and bronchial epithelial cells in response to pro-inflammatory stimuli. FEMS Immunol. Medical Microbiol. 56:233-40.

398.Kindrachuk, J., H. Jenssen, M. Elliott, R. Townsend, A. Nijnik, S.F. Lee, V. Gerdts, L.A.Babiuk, S.A. Halperin and R.E.W. Hancock. 2009. A novel vaccine adjuvant comprised of a synthetic innate defence regulator peptide and CpG oligonucleotide links innate and adaptive immunity. Vaccine 27:4662-4671.

399.Easton, D.M., A. Nijnik, M.L. Mayer, and R.E.W. Hancock. 2009. Potential of immunomodulatory host defence peptides as novel anti-infectives. Trends in Biotechnol. 27:582-590.

400.Mookherjee, N., D.N.D. Lippert, P. Hamill, R. Falsafi, A. Nijnik, J. Kindrachuk, J. Pistolic, J. Gardy, P. Miri, M. Naseer, L.J. Foster, and R.E.W. Hancock. 2009. Intracellular receptor for human host defence peptide LL-37 in monocytes. J. Immunol. 183:2688-2696. Featured at MDLinx.com. http://www.mdlinx.com/internalmdlinx/news-article.cfm/2833610.

401.Schurek, K.N., J.L.M. Sampaio, C.R.V. Kiffer, S. Sinto, C.M.F. Mendes, and R.E.W.Hancock. 2009. Involvement of pmrAB and phoPQ in polymyxin B adaptation and inducible resistance in non-cystic fibrosis clinical isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53:4345 - 4351.

402.Yeung, A.T.Y., E.C.W. Torfs, F. Jamshidi, M. Bains, I. Wiegand, R.E.W. Hancock, and J.Overhage. 2009. Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators including MetR. 2009. J. Bacteriol. 191:5591-5602.

403.Winkler, D.F.H., K. Hilpert, O. Brandt and R.E.W. Hancock. 2009. Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method. Methods Mol Biol. 570:157-74.

404.Nijnik, A., J. Pistolic, A. Wyatt, S. Tam, and R.E.W. Hancock. 2009. Human cathelicidin peptide LL-37 modulates the effects of IFN-Gamma on APC. J. Immunol. 183: 5788 - 5798

405.Yu, H.B., A. Kielczewska, A.Rozek, S. Takenaka, Y. Li, L. Thorson, R.E.W. Hancock, M.M. Guarna, J.R. North, L.J. Foster, O. Donini and B.B. Finlay. 2009. Sequestosome-1/p62 is the key intracellular target of innate defense regulator peptide. J. Biol. Chem. 284:36007-11.

406.Lee, S.M.Y., J.L. Gardy, C.Y. Cheung, T.K.W. Cheung, K.P.Y. Hui1, W.W. Gai1, N.Y. Ip, Y. Guan, R.E.W. Hancock, and J.S.M. Peiris. 2009. Systems-Level Comparison of Host-Responses Elicited by Avian H5N1 and Seasonal H1N1 Influenza Viruses in Primary Human Macrophages. PLOS One 4:e8072. Epub.

407.Amid, C., L.M. Rehaume, K.L. Brown, J.G.R. Gilbert, G. Dougan, R.E.W. Hancock, and J.L. Harrow. 2009. Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome. BMC Genomics 10:606 Epub.

408.Jenssen, H., and R.E.W. Hancock. 2010. Therapeutic Potential of HDPs as Immunomodulatory Agents. Methods in Molecular Biology 20: 329-347.

409.Gong, J.-H. E.F. Nicholls, M.R. Elliott, K.L. Brown, K. Hokamp, F.M. Roche, C.Y Cheung, R. Falsafi, F.S. Brinkman, D.M.E. Bowdish, and R.E.W. Hancock. 2010. G-protein-coupled receptor independent, immunomodulatory properties of chemokine CXCL9. Cellular Immunol. 261:105–113.

410.Hadley, E.B., and R. E.W. Hancock. 2010. Strategies for the identification and advancement of novel cationic antimicrobial peptides. Curr. Topics Medicinal Chem. 8:1872-1881.

411.Nijnik A., L. Madera, S. Ma, M. Waldbrook, M. Elliott, S.C. Mullaly, J. Kindrachuk, H. Jenssen, R.E.W. Hancock. 2010. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J. Immunol. 184:2539 -2550. Reviewed by MDLinx at www.mdlinx.com/allergy-immunology/news-article.cfm/3053419.

412.Brown, K.L., R. Falsafi, W. Kum, P. Hamill, J.L. Gardy, D.J. Davidson, S. Turvey, B.B. Finlay, D.P. Speert and R.E.W. Hancock. 2010. Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity. J. Translational Med. 8:6 Epub.

413.Mayer, M.L., and R.E.W. Hancock. 2010. Cathelicidins link the endocrine and immune systems. (Preview) Cell Host & Microbe 7:257-259.

414.Fjell, C.D., R.E.W. Hancock and H. Jenssen. 2010. Computer-aided design of antimicrobial peptides. Curr. Pharm. Anal. 6:66-75.

415.Li, A.H., S.J. Waddell, J. Hinds, C.A. Malloff, M. Bains, R.E.W. Hancock, W.L. Lam, P.D. Butcher, and R.W. Stokes. 2010. Transcriptional differences between a virulent and an attenuated strain of Mycobacterium tuberculosis infecting murine bone-marrow derived macrophages. PLoS One 5:e11066.

416.Fernandez, L., W.J. Gooderham, M. Bains, J.B. McPhee, I. Wiegand, and R.E.W. Hancock. 2010. Adaptive resistance to the “last hope” antibiotics polymyxin B and colistin in Pseudomonas aeruginosa is mediated by the novel two-component regulatory system ParR-ParS. Antimicrob. Agents Chemother. 54:3372-3382.

417.Lynn, D.J., C. Chan, M. Naseer, M. Yau, R. Lo, A. Sribnaia, G. Ring, J. Que, K. Wee, G.L. Winsor, M.R. Laird, K. Breuer, A.K. Foroushani, F.S.L. Brinkman, and R.E.W. Hancock. 2010. Curating the innate immunity interactome. BMC Systems Biology 4:117 Epub. Highly accessed paper on BMC website.

418.Hilpert, K., B. McLeod, J. Yu, M.R. Elliott, M. Rautenbach, S. Ruden, J. Bürck, C. Muhle-Goll, A.S. Ulrich, S. Keller and R.E.W. Hancock. 2010. Short cationic antimicrobial peptides interact with ATP. Antimicrob. Agents Chemother. 54:4480-4483.

419.Alvarez-Ortega, C., I. Wiegand, J. Olivares, R.E.W. Hancock, and J. Luis Martínez. 2010. Genetic determinants involved in the susceptibility of Pseudomonas aeruginosa to β-lactam antibiotics. Antimicrob. Agents Chemother. 54:4159-4167. Recommended by the Faculty of 1000 Biology.

420.Wieczorek, M., H. Jenssen, J. Kindrachuk, W.R.P. Scott, M. Elliot, K. Hilpert, J.T.J. Cheng, R.E.W. Hancock and S.K. Straus. 2010. Structural studies of a peptide with immune modulating and direct antimicrobial activity. Chem. Biol. 17:970-980.

421.Bommarius, B., H. Jenssen, M. Elliott, J. Kindrachuk, H. Gieren, K.-E. Jaeger, R.E.W. Hancock and D. Kalman. 2010. Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli. Peptides 31:1957-1965.

422.Kazemzadeh-Narbat, M., J. Kindrachuk, K. Duan, H. Jenssen, R.E.W Hancock, and R. Wang. 2010. Antimicrobial peptides on calcium phosphate-coated titanium for the prevention of implant-associated infections. Biomaterials 31:9519-9526.

423.Lee, S.M.Y., R.W.Y. Chan, J.L. Gardy, C.K. Lo, A.D.L. Sihoe,S.S.R. Kang, T.K.W. Cheung, Y. Guan, M.C.W. Chan, R.E.W. Hancock, and J.S.M. Peiris. 2010. Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro. Respir. Res. 11:147.

424.Cheng J.T., J.D. Hale, J. Kindrachuk, H. Jessen, M. Elliott, R.E.W.Hancock and S.K. Straus. 2010. Importance of residue 13 and the C-terminus for the structure and activity of the antimicrobial Peptide aurein 2.2. Biophys J. 99:2926-2935.

425.Van der Weerden, N.L., R.E.W. Hancock, and M.A. Anderson. 2010. Permeabilization of fungal hyphae by the plant defensin NaD1 occurs through a cell wall dependent process. J. Biol. Chem. 285:37513-37520.

426.Nicholls, E.F., L. Madera and R. E. W. Hancock. 2010. Immunomodulators as adjuvants for vaccines and antimicrobial therapy. Ann. NY Acad. Sci. 1213:46-61.

427.Barlow, P.G., P.E. Beaumont, C. Cosseau, A. Mackellar, T.S. Wilkinson, R.E.W. Hancock, C. Haslett, J.R.W. Govan, A.J.N Simpson, and D.J. Davidson. 2010. The human cathelicidin LL-37 preferentially promotes apoptosis of infected airway epithelium. Amer. J. Respir. Cell Mol. Biol. 43:692-702.

428.Fjell, C.D., H. Jenssen, W.A. Cheung, R.E.W. Hancock and A. Cherkasov. 2011. Optimization of antibacterial peptides by genetic algorithms and cheminformatics. Chem. Biol. Drug Design 77:48-56.

429.Winsor, G.L., D.K.W. Lam, L. Fleming, R.Lo, M.D. Whiteside, N.Y. Yu, R.E.W Hancock and F.S.L. Brinkman. 2011. Pseudomonas Genome Database: Improved comparative analysis and population genomics capability for Pseudomonas genomes. Nucl. Acids Res. 39(Database issue):D596-600.

430.Amatngalim, G.D., A. Nijnik, P.S. Hiemstra, and R.E.W. Hancock. 2011. Cathelicidin peptide LL-37 modulates TREM-1 expression and inflammatory responses to microbial compounds. Inflammation, in press.PMID: 20811938.

431.Mader, J.S., M. Marcet-Palacios, R.E.W. Hancock, and C. Bleackley. 2011. The human cathelicidin, LL-37, induces granzyme-mediated apoptosis in cytotoxic T lymphocytes. Exptl. Cell Res. PMID: 21134367.

432.Mader, J.S., C. Ewen, R.E.W. Hancock, and C. Bleackley. 2011. The human cathelicidin, LL-37, induces granzyme-mediated apoptosis. J. Immunother., in press.

433.Mayer, M.L., J.A. Sheridan, C.J. Blohmke, S.E. Turvey, and R.E.W. Hancock. 2011. The Pseudomonas aeruginosa autoinducer 3O-C12 homoserine lactone provokes hyperinflammatory responses from cystic fibrosis airway epithelial cells in a calcium-dependent manner. PLoS One in press.

434.Yeung A.T.Y., M. Bains, and R.E.W. Hancock. 2011. The sensor kinase CbrA is a global regulator that modulates metabolism, virulence and antibiotic resistance in Pseudomonas aeruginosa. J. Bacteriol, in press. PMID: 21169488.

435.Cheng, J.T., J.D. Hale, M. Elliott, R.E.W. Hancock, and S.K. Straus. 2010. The importance of bacterial membrane composition in the structure and function of aurein 2.2 and selected variants. Biochim Biophys Acta. Epub ahead of print. PMID: 21144817.

436.Gracia, A., M. Polewicz, S.A. Halperin, R.E.W. Hancock, A.A. Potter, L.A. Babiuk, and V. Gerdts. 2011. Antibody responses in adult and neonatal BALB/c mice to immunization with novel Bordetella pertussis vaccine formulations. Vaccine in press.

437.Fernandez, L., E.B.M. Breidenstein, and R.E.W. Hancock. 2011. Creeping baselines and adaptive resistance to antibiotics. Drug resistance updates, in press.

438.Spindler, E.C., J.D. Hale, T.H. Giddings Jr, R.E.W. Hancock, and R.T. Gill. 2011. Deciphering the mode of action of a synthetic antimicrobial peptide (Bac8c). Antimicrobial Agents Chemother., in press.

R.E.W. Hancock Laboratory
Updated December 2011