FRENCH PRESS

  1. Position press plunger to the desired volumn marker.
  2. Invert the press onto the stand.
  3. Add the cells. Make sure the level is just below that of the outlet valve, otherwise move the plunger.
  4. Ensure the side valve is open.
  5. Insert the cap all the way.
  6. Close the side valve until finger tight.
  7. Place the cell plunger side-up into the press and lock into place.
  8. Speed: fast. Direction: up. Pump: on.
  9. When the pressure indicator moves, reduce the speed quickly to ensure a pressure of 15,000 psi with the large cell and 200 psi with the small one.
  10. Open the side valve gently until the cells leak out at 11,500 to 13,5000psi. NB: Hold the outlet tube against the wall of the sample container to prevent splashing. (A convenient sample container to use is a 50/12 ml sterile Falcon tube with a slash in the cap for insertion of the outlet tube.)
  11. When the run is completed open the side valve quickly to avoid spurting.
  12. Lower the press and repeat the breakage.
  13. Clean the press with distilled water and ethanol, then with ethanol and distilled water.