Quick Isolation of DNA from Agarose Gels

Categorized: Bacterial Genetics

  1. Run gel as normal in 1X TBE.
  2. Visualize band under LONG wave UV.
  3. Cut band out with razor blade.
  4. Place excised agarose slice in an Eppendorf tube, which has a hole in its bottom (by inserting a hot needle), and which has a small amount of siliconized glass wool covering the opening.
  5. Place this tube inside another Eppendorf tube.
  6. Spin tubes either at 1/2 maximum speed for 15 minutes or, if not possible, full speed for 5 minutes.
  7. Phenol/chloroform the resulting liquid.
  8. Repeat with 1/10 volume 3 M NaAc + 2x volume EtOH.