The following is supplemental data for the publication entitled: "Interaction and cellular localization of the human host defence peptide, LL-37, with lung epithelial cells. (Authors: Y. Elaine Lau, Annett Rozek, Monisha G. Scott, Danika L. Goosney, Donald J. Davidson and R.E.W. Hancock. Publication submitted.)
All array data shown is based on 14K human oligonucleotide expression arrays, which were printed on glass slides using the 70-mer library PRHU04 from Qiagen (Venlo, Netherlands; see www.operon.com for details of the library). These microarray slides were obtained from the Genome BC Array Facility (Vancouver Hospital, Vancouver, BC). cDNA probes were prepared from 2 .g of total cellular RNA by reverse transcription and labeled with either biotin or fluorescein by using a Qiagen LabelStar array kit labeling module as per the manufacturer.s instructions. The cDNA probes were then purified using the cleanup module of the same kit. Microarray slides were subsequently hybridized using the RLS array detection system (Invitrogen/Genicon, San Diego, CA), as per the manufacturer.s instructions. The array hybridization image was captured using GSD-501 RLS detection and imaging instrument (Invitrogen/Genicon). The hybridization signals were then quantified using ArrayVision Version 8.0 (Imaging Research Inc., St. Catherines, ON) and analyzed by GeneSpring (Silicon Genetics, Redwood City, CA). The data was normalized using the 50th percentile parameter of GeneSpring. All results were derived from 3 independent experiments, each involving two technical replicates obtained through dye swapping. All experimental information and procedures were entered into a MIAME compliant database. The parameters used for the table below are a fold change of 3 or greater in cells treated with LL-37 vs untreated cells. The other parameter used was only genes with intensity values of 20 or greater in untreated cells were shown.
All array intensities and ratios.
Centre for Microbial Diseases and Immunity Research
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